Anti-Mouse CD3e Antibody, Clone 145-2C11

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e

Anti-Mouse CD3e Antibody, Clone 145-2C11

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e

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Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e
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Overview

The 145-2C11 antibody reacts with the ~20 kDa CD3ε subunit of the mouse T cell receptor (TCR)/CD3 complex, which is expressed on the surface of circulating mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severed combined immunodeficiency (SCID). The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. The 145-2C11 antibody is not recommended for use with formalin-fixed, paraffin-embedded sections.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).
Subtype
Primary Antibodies
Target Antigen
CD3e
Alternative Names
CD3, CD3epsilon, T3
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Hamster
Cell Type
T Cells
Species
Mouse
Application
Cell Isolation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
145-2C11
Gene ID
12501
Isotype
IgG1, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488 (filled histogram) or an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (filled histogram) or an Armenian hamster IgG PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, followed by an anti-hamster IgG antibody, FITC (filled histogram), or a hamster (Armenian) IgG isotype control antibody followed by an anti-hamster IgG antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (filled histogram) or an Armenian hamster IgG APC isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin followed by streptavidin (SAV) APC (filled histogram) or an Armenian hamster IgG biotin isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC (filled histogram) or an Armenian hamster IgG FITC isotype control antibody (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5 (filled histogram) or an Armenian hamster IgG isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60015PS.1, 60015PS
Lot #
All
Language
English
Catalog #
60015AZ.1, 60015AZ
Lot #
All
Language
English
Catalog #
60015PE.1, 60015PE
Lot #
All
Language
English
Catalog #
60015BT.1, 60015BT
Lot #
All
Language
English
Catalog #
60015FI.1, 60015FI
Lot #
All
Language
English
Catalog #
60015
Lot #
All
Language
English
Catalog #
60015AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015PS.1, 60015PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015AZ.1, 60015AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015PE.1, 60015PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015BT.1, 60015BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015FI.1, 60015FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60015AD
Lot #
All
Language
English