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Anti-Mouse CD3e Antibody, Clone 145-2C11

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e

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Anti-Mouse CD3e Antibody, Clone 145-2C11

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e

From: 83 USD
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Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e
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Overview

The 145-2C11 antibody reacts with the ~20 kDa CD3ε subunit of the mouse T cell receptor (TCR)/CD3 complex, which is expressed on the surface of circulating mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severed combined immunodeficiency (SCID). The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. The 145-2C11 antibody is not recommended for use with formalin-fixed, paraffin-embedded sections.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).
Subtype
Primary Antibodies
Target Antigen
CD3e
Alternative Names
CD3, CD3epsilon, T3
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Hamster
Cell Type
T Cells
Species
Mouse
Application
Cell Isolation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
145-2C11
Gene ID
12501
Isotype
IgG1, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488 (filled histogram) or an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (filled histogram) or an Armenian hamster IgG PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, followed by an anti-hamster IgG antibody, FITC (filled histogram), or a hamster (Armenian) IgG isotype control antibody followed by an anti-hamster IgG antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (filled histogram) or an Armenian hamster IgG APC isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin followed by streptavidin (SAV) APC (filled histogram) or an Armenian hamster IgG biotin isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC (filled histogram) or an Armenian hamster IgG FITC isotype control antibody (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5 (filled histogram) or an Armenian hamster IgG isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC
Catalog #
60015FI, 60015FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5
Catalog #
60015PS.1, 60015PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, PE
Catalog #
60015PE, 60015PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin
Catalog #
60015BT, 60015BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11
Catalog #
60015
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488
Catalog #
60015AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, APC
Catalog #
60015AZ, 60015AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC
Catalog #
60015FI, 60015FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5
Catalog #
60015PS.1, 60015PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, PE
Catalog #
60015PE, 60015PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin
Catalog #
60015BT, 60015BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11
Catalog #
60015
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488
Catalog #
60015AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD3e Antibody, Clone 145-2C11, APC
Catalog #
60015AZ, 60015AZ.1
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (4)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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