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Anti-Rat CD90 Antibody, Clone OX-7

Mouse monoclonal IgG1 antibody against mouse, rat, guinea pig CD90/CD90.1

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Anti-Rat CD90 Antibody, Clone OX-7

Mouse monoclonal IgG1 antibody against mouse, rat, guinea pig CD90/CD90.1

From: 122 USD
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Mouse monoclonal IgG1 antibody against mouse, rat, guinea pig CD90/CD90.1
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Overview

The OX-7 antibody reacts with rat CD90 (Thy-1) and mouse The OX-7 antibody reacts with rat CD90 (Thy-1) and mouse CD90.1 (Thy-1.1), the latter being an allelic form of CD90 expressed by mouse strains AKR/J, PL, and FVB/N. The OX-7 antibody does not react with CD90.2, which is expressed by many mouse strains, including CBA and BALB/c. CD90 is a GPI-linked membrane glycoprotein and member of the immunoglobulin superfamily. The 25 kDa core protein is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25 - 37 kDa). In the rat, CD90 is expressed by several cell types, including hematopoietic stem cells, immature B cells, thymocytes and neurons. In mouse strains expressing CD90.1, it is found on early-stage hematopoietic cells in the bone marrow, thymocytes, and circulating mature T cells. The OX-7 antibody has been reported to induce leukocyte activation, glomerular nephritis, apoptosis in glomerular mesangial cells, and vascular permeability.

This antibody clone has been verified for purity assessments of cells isolated from compatible mouse strains with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851).
Subtype
Primary Antibodies
Target Antigen
CD90/CD90.1
Alternative Names
Thy-1, Thy-1.1
Reactive Species
Guinea Pig, Mouse, Rabbit, Rat
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
T Cells
Species
Mouse, Other, Rat
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
OX-7
Gene ID
21838/24832
Isotype
IgG1, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ) (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, Biotin, followed by SAV APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin, followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of Sprague-Dawley rat brain cells labeled with Anti-Rat CD90 Antibody, Clone OX-7, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram). (B) Flow cytometry analysis of Sprague-Dawley rat thymocytes labeled with Anti-Rat CD90 Antibody, Clone OX-7, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, APC
Catalog #
60024AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488
Catalog #
60024AD, 60024AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7
Catalog #
60024
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, Biotin
Catalog #
60024BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, FITC
Catalog #
60024FI, 60024FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX­7, PE
Catalog #
60024PE, 60024PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, APC
Catalog #
60024AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, Alexa Fluor® 488
Catalog #
60024AD, 60024AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7
Catalog #
60024
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, Biotin
Catalog #
60024BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, FITC
Catalog #
60024FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX­7, PE
Catalog #
60024PE, 60024PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Rat CD90 Antibody, Clone OX-7, FITC
Catalog #
60024FI.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

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Reliable Antibodies for Your Research
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Tools for Your Immunology Research
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Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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