Anti-Human CD45 Antibody, Clone 2D1

Mouse monoclonal IgG1 antibody against human CD45

More Views

Anti-Human CD45 Antibody, Clone 2D1, FITC

Mouse monoclonal IgG1 antibody against human CD45, FITC-conjugated

100 Tests
Catalog #60123FI
443 USD

Anti-Human CD45 Antibody, Clone 2D1, FITC

Mouse monoclonal IgG1 antibody against human CD45, FITC-conjugated

25 Tests
Catalog #60123FI.1
201 USD

Overview

The 2D1 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of hematopoietic stem and progenitor cells and mature blood cells, except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell lineages. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains, one of which is enzymatically active, that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, and 6) encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 - 240 kDa.
Subtype:
Primary Antibodies
Target Antigen:
CD45
Reactive Species:
Human
Conjugation:
FITC
Host Species:
Mouse
Cell Type:
Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Leukocytes; Lymphocytes; Myeloid Cells
Application:
Cell Isolation; Flow Cytometry
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(3)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor CellEnrichment Kit

Figure 1. Data for FITC Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone 2D1, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.