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The 12G5 antibody reacts with CD184 (CXCR4 or fusin), an ~45 kDa seven transmembrane G-protein-coupled receptor expressed on the surface of a broad range of cells, including T and B cells, dendritic cells, monocytes, macrophages, granulocytes, platelets, endothelial and epithelial cells, astrocytes and neurons. At least two isoforms of CD184 are generated by alternate mRNA splicing. Ligands for CD184 include the CXC chemokine SDF-1 and ubiquitin. CD184 has functional roles in mediating chemotaxis, B cell lymphopoiesis and myelopoiesis, cardiogenesis, vascularization and neuronal guidance. CD184 acts as a co-receptor for infection by some T cell-tropic isolates of HIV-1 and as an alternative receptor for CD4-independent infection by HIV-2; functions that are blocked by binding of the 12G5 antibody. The antibody also partially inhibits SDF-1-induced chemotaxis and calcium influx. In differentiation studies, CD184 is used along with other markers such as CD117 to monitor the emergence of definitive endoderm.
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram).
Figure 2. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram).
(B) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (Catalog #60087AZ) and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE. The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184.
(C) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE.
Figure 4. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, followed by anti-mouse IgG, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by anti-mouse IgG, FITC (solid line histogram).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
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Mouse monoclonal IgG2a, kappa isotype control antibody
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Anti-Human CD184 (CXCR4) Antibody, Clone 12G5
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
