Anti-Human CD184 (CXCR4) Antibody, Clone 12G5

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)

Anti-Human CD184 (CXCR4) Antibody, Clone 12G5

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)

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Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)
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Overview

The 12G5 antibody reacts with CD184 (CXCR4 or fusin), an ~45 kDa seven transmembrane G-protein-coupled receptor expressed on the surface of a broad range of cells, including T and B cells, dendritic cells, monocytes, macrophages, granulocytes, platelets, endothelial and epithelial cells, astrocytes and neurons. At least two isoforms of CD184 are generated by alternate mRNA splicing. Ligands for CD184 include the CXC chemokine SDF-1 and ubiquitin. CD184 has functional roles in mediating chemotaxis, B cell lymphopoiesis and myelopoiesis, cardiogenesis, vascularization and neuronal guidance. CD184 acts as a co-receptor for infection by some T cell-tropic isolates of HIV-1 and as an alternative receptor for CD4-independent infection by HIV-2; functions that are blocked by binding of the 12G5 antibody. The antibody also partially inhibits SDF-1-induced chemotaxis and calcium influx. In differentiation studies, CD184 is used along with other markers such as CD117 to monitor the emergence of definitive endoderm.
Subtype
Primary Antibodies
Target Antigen
CD184 (CXCR4)
Alternative Names
C-X-C chemokine receptor type 4, CXCR4, fusin, HM89, HUMSTR, LAP3, LCR1, LESTR, NPYY3R, NPY3R, SDF-1 receptor, WHIM
Reactive Species
African Green Monkey, Baboon, Chimpanzee, Cynomolgus, Human, Rhesus, Sooty Mangabey
Conjugation
APC, Biotin, PE, Unconjugated
Host Species
Mouse
Cell Type
Endoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human, Non-Human Primate
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Area of Interest
Immunology, Stem Cell Biology
Clone
12G5
Gene ID
7852
Isotype
IgG2a, kappa

Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (Catalog #60087AZ) and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE. The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184. (C) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE.

Data for Unconjugated

Figure 4. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, followed by anti-mouse IgG, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by anti-mouse IgG, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60089AZ, 60089AZ.1
Lot #
All
Language
English
Catalog #
60089BT
Lot #
All
Language
English
Catalog #
60089PE.1, 60089PE
Lot #
All
Language
English
Catalog #
60089
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60089AZ, 60089AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60089BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60089PE.1, 60089PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60089
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications