EasySep™ Human CD11b Positive Selection and Depletion Kit

Immunomagnetic positive selection and cell depletion kit

EasySep™ Human CD11b Positive Selection and Depletion Kit

Immunomagnetic positive selection and cell depletion kit

From: 695 USD
Catalog #
100-0742_C
Immunomagnetic positive selection and cell depletion kit

Product Advantages

• Fast and easy-to-use
• Up to 99% purity
• No columns required

What's Included

  • EasySep™ Human CD11b Positive Selection and Depletion Kit (Catalog #100-0742)
    • EasySep™ Human CD11b Positive Selection Depletion Cocktail, 0.75 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • RoboSep™ Human CD11b Positive Selection and Depletion Kit (Catalog #100-0744)
    • EasySep™ Human CD11b Positive Selection Depletion Cocktail, 0.75 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Tips (Catalog #20125)

Overview

Isolate or deplete CD11b+ cells from human peripheral blood mononuclear cells (PBMCs) or leukapheresis samples with the EasySep™ Human CD11b Positive Selection and Depletion Kit. The CD11b+ cells are labeled with antibodies and magnetic particles and separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. The desired cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

CD11b is a commonly used myeloid cell marker and expressed on the cell surface of leukocytes including monocytes, macrophages, neutrophils, and natural killer (NK) cells.

Learn more about how EasySep™ technology works.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Macrophages, Monocytes, NK Cells
Species
Human
Sample Source
Leukapheresis, Peripheral Blood
Selection Method
Depletion, Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0742
Lot #
All
Language
English
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0742
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0742
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0744
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Typical EasySep™ Human CD11b+ Positive Selection Profile

Figure 1. Typical EasySep™ Human CD11b+ Positive Selection Profile

Starting with human PBMCs, the CD11b+ cell content of the isolated fraction is typically 98.6 ± 1.4% after positive selection and 1.7 ± 1.2% after depletion (mean ± SD using the EasySep™ magnet). In the above example, the frequencies of CD11b+ cells in the start, final isolated, and final depleted fractions are 47.8%, 99.4%, and 1.6%, respectively.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.