EasySep™ Human CD11b Positive Selection and Depletion Kit
Immunomagnetic positive selection and cell depletion kit
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Overview
Use this kit to label CD11b+ cells with antibodies and magnetic particles. The cells are then subsequently separated—without the use of columns—using an EasySep™ magnet. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction can be retained for subsequent applications.
This kit targets human CD11b, a subunit of the integrin macrophage-1 antigen (Mac-1) receptor commonly used as a myeloid cell marker. CD11b is expressed on immune cells such as monocytes, macrophages, neutrophils, and NK cell subsets, and is known to play a role in regulating cell adhesion, migration, and phagocytosis (Khan SQ et al. Front Med, 2018).
Learn more about how immunomagnetic EasySep™ technology works.
Data Figures
Figure 1. Typical EasySep™ Human CD11b+ Positive Selection Profile
Starting with human PBMCs, the CD11b+ cell content of the isolated fraction is typically 98.6 ± 1.4% after positive selection and 1.7 ± 1.2% after depletion (mean ± SD using the EasySep™ magnet). In the above example, the frequencies of CD11b+ cells in the start, final isolated, and final depleted fractions are 47.8%, 99.4%, and 1.6%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
