Human Mesenchymal Stem Cell qPCR Array

For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages
Catalog #
07541_C
For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages
From: 179 USD
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Required Products
  1. qPCR Master Mix Kit|07516
    qPCR Master Mix Kit

    For probe-based assays and arrays

  2.  STEMscript™ cDNA Synthesis Kits|79003
    STEMscript™ cDNA Synthesis Kits

    For reverse transcription of RNA before qPCR or RNA sequencing

  3. Total RNA Purification Kit
    Total RNA Purification Kit

    For purification of RNA from cells

  4. Axygen® Sealing Film for Real-Time PCR (qPCR)
    Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)

    Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)

Overview

The Human Mesenchymal Stem Cell Quantitative Polymerase Chain Reaction (qPCR) Array is designed for characterization of human mesenchymal stem and progenitor cells (MSCs) and their differentiated chondrogenic, adipogenic, or osteogenic progeny. MSCs are self-renewing, multipotent precursors found in the adherent fraction of bone marrow and stromal compartments in multiple tissues. MSCs can be expanded in vitro to generate mesenchymal stromal cell cultures, which, under appropriate conditions, can differentiate into adipocytes, chondrocytes, and osteoblasts. This qPCR array provides the gene expression profile of MSCs and their trilineage derivatives following in vitro differentiation. Genes were selected based on their demonstrated differential expression in MSCs (Silva et al.) or in MSC-derived chondrogenic (Herlofsen et al.; Yoo et al.), adipogenic (Hung et al.; Menssen et al.), or osteogenic lineage cells (Kulterer et al.).

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

This qPCR array contains validated primers and probes for detection of 90 genes whose expression is correlated with MSCs or their differentiated derivatives, as well as six endogenous (housekeeping) control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Additional gene information and a qPCR analysis tool are available at www.stemcell.com/qPCRanalysis
Components
  • Human Mesenchymal Stem Cell qPCR Array (Catalog #07541)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Mesenchymal Stem Cell qPCR Array (Catalog #07542)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Mesenchymal Stem Cell qPCR Array (Catalog #07543)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Mesenchymal Stem Cell qPCR Array (Catalog #07544)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
Cell Type
Adipocytes, Chondrocytes, Mesenchymal Stem and Progenitor Cells, Osteoblasts
Species
Human
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Mesenchymal Stem Cell qPCR Array
Catalog #
07541, 07543
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human Mesenchymal Stem Cell qPCR Array
Catalog #
07541, 07543
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human Mesenchymal Stem Cell qPCR Array
Catalog #
07541, 07543
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human Mesenchymal Stem Cell qPCR Array
Catalog #
07541, 07543
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human Mesenchymal Stem Cell qPCR Array
Catalog #
07541, 07543
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human Mesenchymal Stem Cell qPCR Array
Catalog #
07541, 07543
Lot #
All
Language
English

Educational Materials (1)

Brochure
qPCR Arrays for Cell Characterization

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Human Mesenchymal Stem Cell qPCR Array Plate Configuration

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