Overview
This kit is used for selection and cloning applications where the G418 antibiotic is used as the selective agent. ClonaCell™-TCS Medium is a serum-containing, methylcellulose-based semi-solid medium that can be used for selection and cloning of a variety of suspension-adapted and adherent cell lines, such as BHK-1, CHO-K1, CHO-S, CHO-DG44, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA). The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium.
Advantages:
• Supports high cloning efficiency and robust colony formation for a variety of cell lines such as: CHO-K1, CHO-S, HEK-293, B16F-10, BaF/3, BHK-1, FD-5, Jurkat Daudi, Molt-4, UT-7, K562
Components:
- ClonaCell™-TCS Medium, 80 mL (Catalog #03814)
- G418, 250 mg (Catalog #03812)
Subtype:
Specialized Media
Cell Type:
CHO Cells; HEK-293; Other
Species:
Mouse; Other
Application:
Cell Culture; Semi-Solid Cloning
Brand:
ClonaCell
Area of Interest:
Antibody Development; Cell Line Development
Scientific Resources
Product Documentation
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(1)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.