Human Recombinant EPO (HEK293-expressed)

Erythropoietin, Fc tag

Human Recombinant EPO (HEK293-expressed)

Erythropoietin, Fc tag

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Erythropoietin, Fc tag
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Overview

The principal physiological regulator of erythropoiesis, Erythropoietin (EPO) is a glycoprotein growth factor that is produced primarily in the kidney in response to hypoxia or anemia. EPO promotes erythropoiesis by binding to a homodimeric cell surface receptor that activates JAK2/STAT5, PI3K/AKT, and MAPK pathways, and stimulates the proliferation and differentiation of erythroid progenitor cells (Jelkmann. Transfus Med Hemother, 2013; Kuhrt & Wojchowski. Blood, 2015). At the carboxy terminus, Human Recombinant EPO (HEK293-expressed) contains a human IgG1 Fc tag.
Subtype
Cytokines
Alternative Names
Epoetin, Erythropoietin, EP
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology
Purity
≥ 92%

Data Figures

Figure 1. Biological Activity and Molecular Mass of Human Recombinant EPO (HEK293-expressed)

(A) The biological activity of Human Recombinant EPO (HEK293-expressed) was tested by its ability to promote the proliferation of TF-1 cells. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is ≤ 8 ng/mL. (B) Human Recombinant EPO (HEK293-expressed) was resolved with SDS-PAGE under reducing conditions. Human Recombinant EPO has a predicted molecular mass of 45.1 kDa. The recombinant protein migrates with an apparent molecular mass of 60 - 65 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1716, 100-1717
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1716
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1717
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.