PD0325901

MEK/ERK pathway inhibitor; Inhibits MEK

PD0325901

MEK/ERK pathway inhibitor; Inhibits MEK

From: 131 USD
Catalog #
72182_C
MEK/ERK pathway inhibitor; Inhibits MEK

Overview

PD0325901 is a selective, cell permeable inhibitor of the MEK/ERK pathway that inhibits the activation and downstream signaling of MEK. It is an extremely potent inhibitor, suppressing the phosphorylation of ERK in C26 cells at very low concentrations (IC₅₀ = 0.33 nM) (Bain et al., Barrett et al.).

MAINTENANCE AND SELF-RENEWAL
· Maintains undifferentiated mouse embryonic stem (ES) cells, in combination with CHIR99021, in the absence of LIF (Ying et al.).
· Allows derivation and maintenance of rat ES cells (Buehr et al., Li P et al.).

REPROGRAMMING
· Add at the later stages of reprogramming to select for and expand fully reprogrammed mouse induced pluripotent (iPS) cells (Shi et al., Silva et al.).
· Increases the efficiency of reprogramming human somatic cells to iPS cells, in combination with SB431542 and Thiazovivin (Lin et al.).
· Promotes reprogramming of human somatic cells to iPS cells using only a single factor, OCT4 (Zhu et al.).
· Generates mouse-like or “ground state” iPS cells from human and rat somatic cells, in combination with CHIR99021 and A83-01 (Li W et al.).

Alternative Names
Not applicable
Cell Type
Pluripotent Stem Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Maintenance, Reprogramming
Area of Interest
Stem Cell Biology
CAS Number
391210-10-9
Chemical Formula
C₁₆H₁₄F₃IN₂O₄
Molecular Weight
482.2 g/mol
Purity
≥ 98%
Pathway
MEK/ERK
Target
MEK

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
PD0325901
Catalog #
72182, 72184, 100-0248
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
PD0325901
Catalog #
72182, 72184
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
PD0325901
Catalog #
100-0248
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications (10)

Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Zhu S et al. Cell stem cell 2010 DEC
A chemical platform for improved induction of human iPSCs. Lin T et al. Nature methods 2009 NOV

Abstract

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Li W et al. Cell stem cell 2009 JAN

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