Human Recombinant Shh (C24II)

Sonic hedgehog
Catalog #
78065_C
Sonic hedgehog
From: 154 EUR

Overview

Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins that has a prominent role in patterning during early mammalian development (Ho & Scott). It is expressed during early embryogenesis in a variety of tissues (Finco et al.). During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocytes precursors and neural crest cells and stimulates proliferation, differentiation and axon growth (Ho & Scott; Martí). Shh also regulates T cell differentiation and activation (Crompton et al.). Recombinant human Shh (C24II; rhShh) is a fully biologically active molecule.
Subtype
Cytokines
Alternative Names
HHG-1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic hedgehog, TPT, TPTPS
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
19.7 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant Shh (C24II)
Catalog #
78065, 78065.1, 78065.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant Shh (C24II)
Catalog #
78065, 78065.1, 78065.2
Lot #
All
Language
English

Educational Materials (1)

Brochure
Cytokines, Chemokines and Growth Factors

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant Shh (C24II) was tested by its ability to promote alkaline phosphatase production of CCL-226 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example 0.7 μg/mL.
(B) 4 μg of Human Recombinant Shh (C24II) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Shh (C24II) has a predicted molecular mass of 19.7 kDa.

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