• Compare Products
Menu
Account
Until June 18, enjoy savings to support your research with our latest promotion!   Get Promo Codes >

EasySep™ Mouse Memory CD4+ T Cell Isolation Kit

Immunomagnetic negative selection of untouched mouse memory CD4+ T cells (CD4+CD62LhighCD44high/int central memory and CD4+CD62L-CD44high/int effector memory) from single-cell suspensions of mouse splenocytes or other tissues

Loading...
 

EasySep™ Mouse Memory CD4+ T Cell Isolation Kit

Immunomagnetic negative selection of untouched mouse memory CD4+ T cells (CD4+CD62LhighCD44high/int central memory and CD4+CD62L-CD44high/int effector memory) from single-cell suspensions of mouse splenocytes or other tissues

From: 805 USD
Catalog #
(Select a product)
Immunomagnetic negative selection of untouched mouse memory CD4+ T cells (CD4+CD62LhighCD44high/int central memory and CD4+CD62L-CD44high/int effector memory) from single-cell suspensions of mouse splenocytes or other tissues
Add to Wish List

Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 96% purity

  • Untouched, viable cells

What's Included

  • EasySep™ Mouse Memory CD4+ T Cell Isolation Kit (Catalog #19767)
    • EasySep™ Mouse Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 2 x 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18730), 0.2 mL
  • RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit (Catalog #19767RF)
    • EasySep™ Mouse Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 2 x 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18730), 0.2 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasyEights™ EasySep™ Magnet
    EasyEights™ EasySep™ Magnet

    Multiple sample processing magnet for column-free immunomagnetic cell separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Easily and efficiently isolate highly purified mouse memory CD4+ T cells (CD4+CD62LhighCD44high/int central memory and CD4+CD62L-CD44high/int effector memory) from single-cell suspensions of mouse splenocytes or other tissue samples. Use immunomagnetic negative selection, with the EasySep™ Mouse Memory CD4+ T Cell Isolation Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. Non-memory CD4+ T cells as well as other unwanted cells expressing the following markers are targeted for removal: CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ. The magnetically labeled cells are then separated from the untouched desired mouse memory CD4+ T cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation in as little as 27.5 minutes, the desired cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Mouse
Sample Source
Other, Spleen
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Typical EasySep™ Mouse Memory CD4+ T Cell Isolation Profile

Figure 1. Typical EasySep™ Mouse Memory CD4+ T Cell Isolation Profile

Starting with mouse splenocytes, the memory CD4+ T cell (CD4+CD62L high CD44 high/int central memory and CD4+CD62L-CD44 high/int effector memory) content of the isolated fraction typically ranges from 78 - 96%. In the example above, the final purities of the start and isolated fractions are 5.3% and 90.2%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
1000157900 or higher
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
1000157900 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse Memory CD4+ T Cell Isolation Kit
Catalog #
19767
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Mouse Cell Isolation
Brochure
Mouse Cell Isolation
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Streamline Your Mouse T Cell Research from Isolation to Expansion
30:18
Webinar
Streamline Your Mouse T Cell Research from Isolation to Expansion
Mouse Immune Cell Isolation Course
On-Demand Training
Mouse Immune Cell Isolation Course

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

Which cell separation kits are compatible with the EasyPlate™ EasySep™ magnet?

The EasyPlate™-compatible human EasySep™ kits are:
19051 (T Cells), 19052 (CD4 T cells), 19157 (Memory CD4 T Cells), 19053 (CD8 T Cells), 19054 (B Cells), 19254 (Naïve B cells), 19055 (NK Cells), 19058 (Monocytes without CD16 depletion), 19059 (Monocytes)

The EasyPlate™-compatible mouse EasySep™ kits are:
19751 (T Cells), 19752 (CD4 T cells), 19753 (CD8 T Cells), 19754 (B Cells), 19755 (NK Cells - please contact Tech Support)

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

Currently, EasySep™ Streptavidin RapidSphere™ kits are only available for negative selection and work by targeting and removing unwanted cells.

How does the separation work?

Streptavidin RapidSphere™ magnetic particles are crosslinked to unwanted cells using biotinylated antibodies. When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a new tube.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Yes. Desired cells are unlabeled and ready to use in downstream applications, such as FACS analysis.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

Publications (1)

T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis. R. J. Napier et al. Nature communications 2020

Abstract

Mutations in nucleotide-binding oligomerization domain-containing protein 2 (NOD2) cause Blau syndrome, an inflammatory disorder characterized by uveitis. The antimicrobial functions of Nod2 are well-established, yet the cellular mechanisms by which dysregulated Nod2 causes uveitis remain unknown. Here, we report a non-conventional, T cell-intrinsic function for Nod2 in suppression of Th17 immunity and experimental uveitis. Reconstitution of lymphopenic hosts with Nod2-/- CD4+ T cells or retina-specific autoreactive CD4+ T cells lacking Nod2 reveals a T cell-autonomous, Rip2-independent mechanism for Nod2 in uveitis. In naive animals, Nod2 operates downstream of TCR ligation to suppress activation of memory CD4+ T cells that associate with an autoreactive-like profile involving IL-17 and Ccr7. Interestingly, CD4+ T cells from two Blau syndrome patients show elevated IL-17 and increased CCR7. Our data define Nod2 as a T cell-intrinsic rheostat of Th17 immunity, and open new avenues for T cell-based therapies for Nod2-associated disorders such as Blau syndrome.
View publication
View All Publications

Related Products

Related Products

Labeling Antibodies

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

Copyright © 2026 by STEMCELL Technologies. All rights reserved.