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Isolate Mouse Immune Cells

Use EasySep™ for highly purified cells in as little as 15 minutes

You can now isolate mouse immune cells in as little as 15 minutes with our next-generation EasySep™ mouse cell isolation kits, featuring our RapidSpheres™ technology.

That's a time savings of up to 45 minutes per separation compared to other negative selection kits. Isolated mouse B cells, pan-B cells, T cells, CD4+ T cells, CD8+ T cells, naïve CD4+ T cells, memory CD4+ T cells, naïve CD8+ T cells, NK cells, monocytes and hematopoietic progenitor cells are untouched, highly purified and ready for immediate downstream use.

Why Use EasySep™?

  • Isolate highly purified mouse immune cells in as little as 15 minutes.
  • With no columns or washes, the simple EasySep™ pour-off is gentle on your cells.
  • Achieve purities of up to 99%.
  • Process up to 16 samples over a wide range of sample sizes at the same time.
  • Without having to buy columns, save money on consumables.

EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save?

What are immunologists doing with all the time they're saving with STEMCELL Technologies' new 15-minute EasySep™ mouse cell isolation kits? How much time will you save?

Mouse Immune Cell Isolation: Head-to-Head Protocol Comparison

This video presents a head-to-head, in-lab comparison of two mouse immune cell isolation protocols: STEMCELL Technologies' 15-minute EasySep™ cell isolation kit and a traditional column-based cell isolation kit.

How Does EasySep™ Work?

Typical EasySep™ Mouse Cell Isolation Protocol (Negative Selection)



High Purity of Isolated Cells

Figure 1. Typical EasySep™ Mouse B Cell Isolation Profile.

Starting with mouse splenocytes, the B cell content of the isolated fraction typically ranges from 94% - 98%.

Figure 2. Typical EasySep™ Mouse CD4+ T Cell Isolation Profile.

Starting with mouse splenocytes, the CD4+ T cell content of the isolated fraction typically ranges from 89% - 96%.

Table 1. Typical purities for specific cell types isolated using EasySep™ Mouse Cell Isolation Kits

Cell Type Typical Purity
B cells 94 - 98%
Pan-B cells 91 - 98%
T cells 92 - 99%
CD4+ T cells 89 - 96%
CD8+ T cells 87 - 95%
Naïve CD4+ T cells 90 - 96%
Memory CD4+ T cells 78 - 96%
Naïve CD8+ T cells 92 - 98%
NK cells 67 - 89%
Monocytes 93 - 96%
Hematopoietic progenitor cells 60 - 84%