Isolate Mouse Immune Cells
Use EasySep™ for highly purified cells in as little as 15 minutes
You can now isolate mouse immune cells in as little as 15 minutes with our next-generation EasySep™ mouse cell isolation kits, featuring our RapidSpheres™ technology.
That's a time savings of up to 45 minutes per separation compared to other negative selection kits. Isolated mouse B cells, pan-B cells, T cells, CD4+ T cells, CD8+ T cells, naïve CD4+ T cells, memory CD4+ T cells, naïve CD8+ T cells, NK cells, monocytes and hematopoietic progenitor cells are untouched, highly purified and ready for immediate downstream use.
Why Use EasySep™?
- Isolate highly purified mouse immune cells in as little as 15 minutes.
- With no columns or washes, the simple EasySep™ pour-off is gentle on your cells.
- Achieve purities of up to 99%.
- Process up to 16 samples over a wide range of sample sizes at the same time.
- Without having to buy columns, save money on consumables.
EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save?
What are immunologists doing with all the time they're saving with STEMCELL Technologies' new 15-minute EasySep™ mouse cell isolation kits? How much time will you save?
Mouse Immune Cell Isolation: Head-to-Head Protocol Comparison
This video presents a head-to-head, in-lab comparison of two mouse immune cell isolation protocols: STEMCELL Technologies' 15-minute EasySep™ cell isolation kit and a traditional column-based cell isolation kit.
How Does EasySep™ Work?
Typical EasySep™ Mouse Cell Isolation Protocol (Negative Selection)
High Purity of Isolated Cells
Figure 1. Typical EasySep™ Mouse B Cell Isolation Profile.
Starting with mouse splenocytes, the B cell content of the isolated fraction typically ranges from 94% - 98%.
Figure 2. Typical EasySep™ Mouse CD4+ T Cell Isolation Profile.
Starting with mouse splenocytes, the CD4+ T cell content of the isolated fraction typically ranges from 89% - 96%.
Table 1. Typical purities for specific cell types isolated using EasySep™ Mouse Cell Isolation Kits
|Cell Type||Typical Purity|
|B cells||94 - 98%|
|Pan-B cells||91 - 98%|
|T cells||92 - 99%|
|CD4+ T cells||89 - 96%|
|CD8+ T cells||87 - 95%|
|Naïve CD4+ T cells||90 - 96%|
|Memory CD4+ T cells||78 - 96%|
|Naïve CD8+ T cells||92 - 98%|
|NK cells||67 - 89%|
|Monocytes||93 - 96%|
|Hematopoietic progenitor cells||60 - 84%|