Isolate Human Immune Cells
Use EasySep™ for highly purified cells in as little as 8 minutes
Isolate human immune cells in as little as 8 minutes with our EasySep™ human cell isolation kits. Isolated cells are highly purified, functional and ready for immediate downstream use. These fast and easy human cell isolation kits are compatible with a variety of EasySep™ magnets for processing a wide range of sample volumes.
Why Use EasySep™?
- Isolate human immune cells in as little as 8 minutes.
- Obtain viable, functional cells without the need for columns and washes.
- Achieve purities of up to 99%.
- Isolate cells from various sample sources, including whole blood and leukapheresis samples.
How Does EasySep™ Work?
Typical EasySep™ Human Cell Isolation Protocol (Negative Selection)
*Time taken varies depending on the kit and type of magnet used
Fast and Easy Cell Isolation with EasySep™
EasySep™ is a column-free immunomagnetic system for the fast and easy isolation of cells that are immediately ready for downstream applications. Cells are targeted using antibody complexes directed toward specific cell surface antigens, which link target cells to EasySep™ magnetic particles. The sample is placed in an EasySep™ magnet, which retains magnetically labeled cells. Untouched cells can be simply poured or pipetted off into a new tube.
Hear From Current EasySep™ Users
How are immunologists using EasySep™ to push science forward? We asked immunologists about their research, scientific journey, and why they use EasySep™.
Switching your cell separation technology to EasySep™ is easier than you think.
How does EasySep™ Perform?EasySep™ protocols are optimized for the efficient isolation of highly purified immune cells that retain functionality.
High Purity and Recovery
Figure 1. EasySep™ Yields Equivalent or Better Purity and Recovery of Human T Cells Compared to a Column-Based Technology
T cells were isolated by negative selection (Neg Sel) or positive selection (Pos Sel) using EasySep™ or a competitor’s column-based technology. EasySep™ isolation yielded comparable or better purity (A) and recovery (B) to the competitor’s system. Data shown as mean ± SEM; n = 3.
Figure 2. Typical EasySep™ Human T Cell Isolation Profile
Starting with human PBMCs, the T cell content (CD3+) of the isolated fraction is typically 96.7 ± 1.5% (mean ± SD for the purple EasySep™ Magnet).
Table 1. Typical purities for specific cell types isolated using EasySep™ Human Cell Isolation Kits
Isolated Cells Respond Appropriately to Stimuli
Figure 3. T Cells Isolated Using EasySep™ Show an Appropriate Activation Phenotype
Human T cells were isolated from peripheral blood mononuclear cells (PBMCs) by negative selection (Neg Sel) or positive selection (Pos Sel) using EasySep™ or a competitor’s column-based system. Isolated T cells were assessed for CD25 expression immediately after isolation (Day 0) and after 3 days in culture with or without anti-CD3/CD28 stimulation. (A) At Day 0, isolated T cells expressed similar levels of CD25 compared to CD3+ cells in unmanipulated PBMCs. (B) At Day 3, cells remained unactivated in the absence of stimulation, while stimulated cells expressed the activation marker CD25. Data shown as mean ± SEM; n = 3.
Figure 4. Central Memory and Effector Memory CD4+ T Cells Isolated by EasySep™ Produce Cytokines When Stimulated
T cell subsets were isolated using the EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit. Isolated cells were cultured in medium with or without PMA and ionomycin stimulation for 4 hours. T cell subsets were assessed for the expression of IL-2 (A) and IFNγ (B) by intracellular flow cytometry. In the absence of stimulation, T cell subsets produced low levels of IL-2 and IFNγ. (A) When stimulated, central memory (CM) and effector memory (EM) CD4+ T cell subsets showed significantly higher IL-2 expression compared to naïve T cells. (B) EM CD4+ T cells expressed higher levels of IFNγ than CM CD4+ T cells when stimulated. Both CM and EM CD4+ T cell subsets showed higher IFNγ expression than naïve T cells. Data shown as mean ± SEM; n = 3.
Protocols are Carefully Optimized
EasySep™ protocols and reagents are optimized to ensure robust and optimal performance across samples. Cocktails and magenetic particles are titrated to minimize non-specific binding, ensure proper cell labeling, and to avoid epitope blocking.
Figure 5. CD14 Epitopes Are Not Blocked Following Cell Isolation Using EasySep™
Cells were isolated by negative selection (Neg Sel) or positive selection (Pos Sel) methods using EasySep™ or a competitor’s column-based technology. Isolated cells were stained using an anti-CD14 antibody (clone M5E2), and assessed by flow cytometry. EasySep™-isolated cells showed similar levels of CD14 staining (MFI) compared to unprocessed CD14+ cells (Leukapheresis Start). Data shown as mean ± SEM; n = 4 - 5.
Magnetic Cell Separation
Find answers to three commonly asked questions about immunomagnetic cell separation.
Choosing a Positive or Negative Selection Approach
Read more about antibody labeling-based cell separation methods, including immunomagnetic and immunodensity cell separation.