Centrifugation Speed and Time for Cells
Optimal centrifugation conditions are required for specific cell types. This guide provides centrifugation speed, time & temperature for various protocols
Optimal centrifugation procedures are required for specific cell types and samples. Below is a quick guide for the centrifugation speed, time, and temperature that we recommend for use with different protocols.
Centrifugation Procedures for Washing Cells
*Room temperature: 15 - 25 °C
Centrifugation Procedures by Cell Separation Method
Density gradient medium for mononuclear cell isolation
(Or: 1200 x g)
(Or: 20 min)
> 24 hrs old: 20 min
> 24 hrs old: 20 min
Centrifugation Procedures for Processing Neurospheres
Related Resources
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Lymphoprep™ Density Gradient Medium
A cost-effective alternative to Ficoll-Paque™ that can be used with SepMate™ for the isolation of mononuclear cells from blood.
RosetteSep™ Immunodensity Cell Separation
Isolate human cells directly from whole blood during density gradient centrifugation. Combine with SepMate™ for a faster protocol.
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