Human pluripotent stem cells (hPSCs) can be maintained along a spectrum of different pluripotent states. In vitro, different cell culture media with specific combinations of cytokines or small molecules have been shown to maintain cells in either a naïve or primed pluripotent state. Multiple research groups have recently identified different culture conditions capable of shifting and maintaining hPSCs toward the "ground" or "naïve" state and away from the traditional primed state of hPSCs.
Explore the resources below for information on the ground state hPSC research.
Dr. Arwen Hunter, a scientist at STEMCELL Technologies, describes the application of RSeT™ medium to revert and maintain primed hPSCs to ground or naïve-like state. This video was filmed during the Innovation Showcase at ISSCR 2016 in San Francisco.View Now >
Key Applications of Naïve-Like Stem Cells
O'Brien CM et al. (2016) New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells. Stem Cells 35(3):626-640
Liu X et al. (2017) Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming. Nat Methods 14 11 1055-1062
Ward E et al. (2017) Feeder-Free Derivation of Naïve Human Pluripotent Stem Cells. Stem Cells Dev 26(15):1087-1089
Collier AJ et al. (2017) Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States. Cell Stem Cell 20(6):874-890
Varga E et al. (2017) Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD). Stem Cell Res 21:19-22