• Serum-free formulation contains pre-screened quality components for reproducible results
• Facilitates highly efficient reversion to naïve-like state with stable domed morphology, naïve gene expression profiles, and low levels of spontaneous differentiation without the need for exogenous genes
• Maintains naïve-like pluripotency without inclusion of bFGF
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. Schematic of Reversion of Primed to Naïve-Like hPSCs with RSeT™ Feeder-Free
Primed hPSCs are plated as aggregates in mTeSR™1. On day 1, mTeSR™1 is replaced with RSeT™ Feeder-Free, the cultures are transferred to hypoxic conditions, and the medium is exchanged every other day. By day 4 or 5, the colonies are generally large enough to be passaged. During the initial culture in RSeT™ Feeder-Free, colonies expand and begin to adopt a tightly-packed, highly domed morphology characteristic of naïve-like stem cells with smooth and refractive colony edges as early as passage 1.
Figure 2. hPSCs Maintained in RSeT™ Feeder-Free are Reverted to a Naïve-Like State and Express High Levels of Naïve-Associated Genes
(A) A representative image of hPSCs that reverted to a naïve-like state after being cultured in RSeT™ Feeder-Free for 1 passage. During reversion, colonies change from a flat morphology to a domed morphology characteristic of naïve-state hPSCs. (B) Expression of naïve-associated genes (KLF2, KLF4, KLF17, TFCP2L1, STELLA, and DNMT3L) in hPSCs that were reverted to a naïve-like state in RSeT™ Feeder-Free. Expression levels were measured by qPCR and normalized to levels in primed hPSCs.