RSeT™ Feeder-Free Medium

Serum-free medium for naïve-like human pluripotent stem cells
RSeT™ Feeder-Free Medium

Serum-free medium for naïve-like human pluripotent stem cells

500 mL Kit
Catalog # 05975
377 USD

Overview

RSeT™ Feeder-Free Medium is a serum-free cell culture medium used for the reversion of primed human embryonic stem (ES) and induced pluripotent stem (iPS) cells to a naïve-like state and maintains cells in a naïve-like state under hypoxic conditions without bFGF or feeder cells. RSeT™ Feeder-Free Medium was developed under license from the Weizmann Institute of Science. With pre-screened quality components that ensure batch-to-batch consistency, this medium produces robust cultures with features of a naïve-like state such as tightly packed, domed colonies with refractive edges. Key transcripts associated with naïve-like human ES/iPS cells, such as KLF17, KLF2, KLF4, and TFCP2L1, show increased expression in human ES/iPS cells cultured in RSeT™ Feeder-Free Medium. RSeT™ Feeder-Free hPSCs can either be differentiated, or converted back to a primed state by culture in mTeSR™1 and then differentiated. Products that can be used for differentiation include STEMdiff™ Definitive Endoderm Kit (Catalog #05110), STEMdiff™ SMADi Neural Induction Kit (Catalog #08581), and STEMdiff™ Mesoderm Induction Medium (Catalog #05220).
Advantages
• Feeder-independent culture system reduces inherent variability, cost, and burden of feeder preparation
• Serum-free formulation contains pre-screened quality components for reproducible results
• Facilitates highly efficient reversion to naïve-like state with stable domed morphology, naïve gene expression profiles, and low levels of spontaneous differentiation without the need for exogenous genes
• Maintains naïve-like pluripotency without inclusion of bFGF
Components
RSeT™ Feeder-Free Basal Medium, 450 mL
RSeT™ Feeder-Free 10X Supplement, 50 mL
 
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Expansion, Maintenance
Brand
RSeT, TeSR
Area of Interest
Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
RSeT™ Feeder-Free Medium
Catalog #
05975
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RSeT™ Feeder-Free Medium
Catalog #
05975
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RSeT™ Feeder-Free Medium
Catalog #
05975
Lot #
All
Language
English

Educational Materials(2)

Brochure
Products for Human Pluripotent Stem Cells
Scientific Poster
A Feeder-Independent Culture System to Convert and Maintain Human Pluripotent Stem Cells in a Naïve-Like State

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Schematic of Reversion of Primed to Naïve-Like hPSCs with RSeT™ Feeder-Free

Primed hPSCs are plated as aggregates in mTeSR™1. On day 1, mTeSR™1 is replaced with RSeT™ Feeder-Free, the cultures are transferred to hypoxic conditions, and the medium is exchanged every other day. By day 4 or 5, the colonies are generally large enough to be passaged. During the initial culture in RSeT™ Feeder-Free, colonies expand and begin to adopt a tightly-packed, highly domed morphology characteristic of naïve-like stem cells with smooth and refractive colony edges as early as passage 1.

Figure 2. hPSCs Maintained in RSeT™ Feeder-Free are Reverted to a Naïve-Like State and Express High Levels of Naïve-Associated Genes

(A) A representative image of hPSCs that reverted to a naïve-like state after being cultured in RSeT™ Feeder-Free for 1 passage. During reversion, colonies change from a flat morphology to a domed morphology characteristic of naïve-state hPSCs. (B) Expression of naïve-associated genes (KLF2, KLF4, KLF17, TFCP2L1, STELLA, and DNMT3L) in hPSCs that were reverted to a naïve-like state in RSeT™ Feeder-Free. Expression levels were measured by qPCR and normalized to levels in primed hPSCs.

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