Human Pluripotent Stem Cell Naïve State qPCR Array

For characterization of gene expression associated with hPSC naïve or primed states

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Human Pluripotent Stem Cell Naïve State qPCR Array

For characterization of gene expression associated with hPSC naïve or primed states

From: 205 USD
Catalog #
07521_C
For characterization of gene expression associated with hPSC naïve or primed states

What's Included

  • Human Pluripotent Stem Cell Naïve State qPCR Array (Catalog #07521)
    • hPSC Naïve State qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell Naïve State qPCR Array (Catalog #07522)
    • hPSC Naïve State qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell Naïve State qPCR Array (Catalog #07523)
    • hPSC Naïve State qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Pluripotent Stem Cell Naïve State qPCR Array (Catalog #07524)
    • hPSC Naïve State qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Characterize your hPSCs by accurately measuring their gene expression profile to determine their status along the spectrum from naïve to primed pluripotency.

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

The Human Pluripotent Stem Cell (hPSC) Naïve State qPCR Array has validated primers and probes to detect 90 genes preselected for their demonstrated differential expression in naïve state and primed hPSCs and their derivatives, as well as 6 endogenous control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Naïve state hPSCs are self-renewing, retain the properties of the pre-implantation blastocyst, and can differentiate to both embryonic and extra-embryonic lineages. Primed hPSCs are also self-renewing, but they are restricted to differentiation along the three embryonic germ layers. Additional gene information and a qPCR analysis tool are available here.
Cell Type
Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology

Data Figures

Figure 1. Human Pluripotent Stem Cell Naïve State qPCR Array Plate Configuration

Figure 2.

Quantitative PCR analysis of gene expression of H1 hESCs cultured in a naive-like state compared to H1 hESCs cultured under a primed state. Genes associated with higher expression in a naive state than a primed state are shown in light green, genes expected to be more highly expressed in a primed state compared to a naive state are shown in orange and pluripotency associated genes expressed highly in both naive and primed states are shown in yellow. Genes associated with endoderm (red), mesoderm (light blue) ectoderm (dark green) and partially reprogrammed genes (dark blue) in feeder-free versus feeder-dependent tissue culture are also show. Y-axis measures the log change expression difference and X-axis indicates the name of the gene. Experiments were run on a Viia7 Fast (Applied Biosystems) Instrument and data analysis was performed using STEMCELL qPCR analysis program (www.stemcell.com/qPCRanalysis).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
07521
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
07521
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
07521
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
07521
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
07521
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
07521
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

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