Figure 1. Human Pluripotent Stem Cell Naïve State qPCR Array Plate Configuration

Figure 2.

Quantitative PCR analysis of gene expression of H1 hESCs cultured in a naive-like state compared to H1 hESCs cultured under a primed state. Genes associated with higher expression in a naive state than a primed state are shown in light green, genes expected to be more highly expressed in a primed state compared to a naive state are shown in orange and pluripotency associated genes expressed highly in both naive and primed states are shown in yellow. Genes associated with endoderm (red), mesoderm (light blue) ectoderm (dark green) and partially reprogrammed genes (dark blue) in feeder-free versus feeder-dependent tissue culture are also show. Y-axis measures the log change expression difference and X-axis indicates the name of the gene. Experiments were run on a Viia7 Fast (Applied Biosystems) Instrument and data analysis was performed using STEMCELL qPCR analysis program (www.stemcell.com/qPCRanalysis).