Characterize your hPSCs by accurately measuring their gene expression profile to determine their status along the spectrum from naïve to primed pluripotency.

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

The Human Pluripotent Stem Cell (hPSC) Naïve State qPCR Array has validated primers and probes to detect 90 genes preselected for their demonstrated differential expression in naïve state and primed hPSCs and their derivatives, as well as 6 endogenous control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Naïve state hPSCs are self-renewing, retain the properties of the pre-implantation blastocyst, and can differentiate to both embryonic and extra-embryonic lineages. Primed hPSCs are also self-renewing, but they are restricted to differentiation along the three embryonic germ layers. Additional gene information and a qPCR analysis tool are available here