Genome Editing of Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) hold great promise for the study and treatment of human disease owing to their ability to differentiate into many specialized cell types of the human body. The use of genome editing tools to generate specific DNA variants in hPSCs, by either introducing disease-specific mutations or correcting genetic aberrations, provide more accurate modeling of human disease when differentiated into human-derived tissues. Advances in genetic tools such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) or most recently clustered regularly interspaced short palindromic repeat (CRISPR), have improved the ease and efficiency of genome editing in hPSCs.
Explore the resources below to support your genome editing using hPSCs.
View this webinar presentation by Dr. Ashley Watson to learn more about optimized workflows for CRISPR-Cas9 genome editing in difficult-to-manipulate cell types, including human pluripotent stem cells and primary T cells.View Now >
Introduction to Genome Editing
Get a brief overview of genome editing. Find key publications, discover new tools, and listen to webinars from experts in the field.
Wiley E-book: Genome Editing Applications
Learn about next-generation disease modeling using CRISPR, including comprehensive genome editing strategies for complex cell culture models, suggestions for optimizing experimental conditions, and troubleshooting tips.
Panel: Challenges in Ensuring hPSC Quality
Hear global experts discuss key issues impacting the use of human pluripotent stem cells in this series of webinars provided in partnership with Nature Research.