ReproTeSR™ Medium for Reprogramming (2-Component)

Reprogramming medium for human iPS cell induction

ReproTeSR™ Medium for Reprogramming (2-Component)

Reprogramming medium for human iPS cell induction

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Reprogramming medium for human iPS cell induction
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Product Advantages


  • Defined, feeder-free formulation facilitates reproducibly efficient human iPS cell generation

  • Rapid emergence of large colonies with high-quality iPS cell-like morphology facilitates identification and subcloning for easily establishing iPS cell lines

  • Seamlessly integrates with STEMCELL products prior to reprogramming and after iPS cell generation for maintenance and differentiation

What's Included

TeSR™-E7™/ReproTeSR™ Basal Medium, 480 mL
ReproTeSR™ 25X Supplement, 20 mL
 

Overview

ReproTeSR™ (2-Component) is a complete and xeno-free reprogramming medium developed for generating human induced pluripotent stem (iPS) cells from fibroblasts, urine-derived cells, and blood-derived cells such as CD34+ or erythroid precursor cells under feeder-free conditions.

ReproTeSR™ is also available as part of an integrated set of tools that are optimized for reprogramming blood-derived cell types expanded in vitro from peripheral blood, such as CD34+ cells (CD34+ Progenitor Reprogramming Kit, Catalog #05925) or erythroid cells (Erythroid Progenitor Reprogramming Kit, Catalog #05924).
Subtype
Specialized Media
Cell Type
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Reprogramming
Brand
TeSR
Area of Interest
Stem Cell Biology
Formulation Category
Serum-Free, Xeno-Free

Data Figures

Figure 1. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean /- SEM; erythroid cells, n=4; CD34 cells, n=5.

Figure 2. ReproTeSR™ Efficiently Reprograms Fibroblasts

Dermal fibroblasts were transfected with the ReproRNA™-OKSGM vector and reprogrammed under feeder-dependent (standard KOSR-containing hES cell medium on inactivated mouse embryonic fibroblasts (iMEFs)) or feeder-independent conditions (ReproTeSR™ on Corning® Matrigel®). Fibroblasts (passage 4) were reprogrammed with average efficiencies of 0.10 ± 0.06% (hES cell medium) and 0.20 ± 0.07% (ReproTeSR™). Reprogramming efficiency of fibroblasts with ReproRNA™ and ReproTeSR™ is comparable to that reported with Sendai virus. (Schlaeger TM, et al. (2015) Nat Biotechnol 33(1): 58-63.) (n ≥ 6; Data shown are mean ± SD).

Figure 3. Feeder-Free Reprogramming with ReproRNA™-OKSGM Vector and ReproTeSR™ Generates iPS Cell Colonies with Superior Colony Morphology

Representative images of iPS cell colonies were generated using ReproRNA™‑OKSGM and cultured in (A) standard hES cell medium on irradiated mouse embryonic fibroblasts (iMEFs) or (B) ReproTeSR™ on Corning® Matrigel®. iPS cell colonies derived using ReproTeSR™ exhibit more defined borders, compact morphology, and reduced differentiation as compared to the ES cell medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05926
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05926
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05926
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.