Rat Recombinant M-CSF

Macrophage colony-stimulating factor

Rat Recombinant M-CSF

Macrophage colony-stimulating factor

From: 346 USD
Catalog #
78117_C
Macrophage colony-stimulating factor

Overview

Macrophage colony-stimulating factor (M-CSF) is a homodimeric cytokine that belongs to receptor tyrosine kinase subclass III of the receptor tyrosine kinase (RTK) family. M-CSF acts on a CSF-1 receptor tyrosine kinase, which initiates signaling cascades to support cell proliferation and differentiation (Hamilton). M-CSF is produced by endothelial cells, osteoblasts, and during pregnancy by the uterine epithelial cells (Ryan). M-CSF synergizes with other factors to support proliferation and differentiation of multipotent hematopoietic progenitor cells, and regulates proliferation and differentiation of the mononuclear phagocyte progenitor cells to monocytes and macrophages. It also supports survival, proliferation, and function of the differentiated macrophages and regulates differentiation of mononuclear phagocytes to osteoclasts (Pixley & Stanley). M-CSF plays an important role in the implantation of the embryo and early development (Makrigiannakis et al.).
Subtype
Cytokines
Alternative Names
CSF-1, Macrophage colony-stimulating factor
Cell Type
Hematopoietic Stem and Progenitor Cells, Monocytes
Species
Rat
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
18 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Rat Recombinant M-CSF was tested by its ability to promote the proliferation of M-NFS-60 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is less than 2.5 ng/mL. (B) 2 μg of Rat Recombinant M-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78117.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78117.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

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