Human Umbilical Vein Endothelial Cells
Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.
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- ThawSTAR® CFT2 Automated Thawing System
Automated cell thawing system for consistent thawing performance
- EC-Cult™-XF Culture Kit
Culture kit for derivation and proliferation of human endothelial colony-forming cells (ECFCs) and mature endothelial cells
HUVECs are collected using Institutional Review Board (IRB)-approved consent forms and protocols. They can show expression of endothelial markers through later passages, an ability to form tubular networks, as well as uptake of acetylated LDL. HUVECs can be used as an endothelial cell model to study physiological and pathological pathways, such as inflammation, angiogenesis, oxidative stress, hypoxia, and blood vessel formation. With personalized service, custom products, and flexible delivery times, we help you get the cells you need.
Additional documentation and high-resolution HLA typing (Class I and Class II alleles) and CMV status is available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Full product information is provided on the Certificate of Analysis.
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Figure 1. HUVECs Cultured in EC-Cult™-XF Have Cobblestone-Like Morphology
Phase-contrast image of HUVECs cultured in EC-Cult™-XF. Cells grow as monolayers and retain their typical cobblestone-like morphology of endothelial cells.
Figure 2. HUVECs Cultured Long-Term in Complete EC-Cult™-XF Retain Purity of Endothelial Markers.
HUVECs cultured and passaged in complete EC-Cult™-XF medium were compared to HUVECs cultured and passaged in an alternative commercial medium. Both sets of HUVECs were then analyzed by flow cytometry. Cells cultured in EC-Cult™-XF medium had higher retention of the endothelial markers CD31 and CD144 through 19 passages.
Figure 3. HUVECs Can Be Expanded For Greater Passage Numbers in EC-Cult™-XF Than in Alternative Commercial Media
HUVECs were cultured and expanded in EC-Cult™-XF medium or alternative commercial medium (A and B). HUVECs expanded in complete EC-Cult™-XF medium outperformed those in the alternative media, with population doublings continuing to increase beyond passage 16.
Figure 4. HUVECs Form Tubular Networks When Cultured in Complete EC-Cult™-XF Medium
HUVECs, from passage 2 and passage 7, were plated at 10,000 cells/well (96-well plate) in complete EC-Cult™-XF medium and grown for 24 hours on Corning® Matrigel®. Cells were imaged at different time points and shown to form defined tubular branching points as early as after 2 hours of plating.
Figure 5. HUVECs are Functionally Active at Early and Late Passages
The ability of HUVECs to metabolise acetylated LDL was examined by adding labeled acetylated LDL into the cells and incubated for 4-6 hours before imaging using a confocal microscope. Cells were shown to be functionally active and able to incorporate acetylated LDL at early (P2) and late (P7) passage when cultured in EC-Cult™-XF Medium.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
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Human Umbilical Vein Endothelial Cells
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.