Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
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Data and Publications
Figure 1. Cryopreserved Pan-T Cells Secrete IL-2 Upon Activation
T cells freshly isolated from a Leuko Pak (Catalog #70500) using EasySep™ Human T Cell Isolation Kit (Catalog #17951) or cryopreserved Pan-T Cells (Catalog #70024) were cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) and incubated for 48 hours with or without ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971). Freshly isolated and cryopreserved purified T cells secrete similar levels of IL-2 upon activation as measured using the Human IL-2 ELISA Kit (Catalog #02006). *IL-2 concentration of control in culture was lower than the limit of detection.