EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

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EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

1 x 109 cells
Catalog #100-0047
737 USD

Required Products

Overview

The EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit is designed to isolate CD271⁺ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.

Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages:
⦁ Highly purified human PSC-derived neural crest cells isolated in less than 20 minutes
⦁ No-wash removal of EasySep™ Releasable RapidSpheres™
⦁ Compatible with downstream differentiation protocols
Components:
  • EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Cocktail, 2 x 1.0 mL
  • EasySep™ Isolation Cocktail Enhancer, 1.0 mL
  • EasySep™ Release Buffer (Concentrate), 3 x 1.0 mL
  • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1.0 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
Subtype:
Cell Isolation Kits
Cell Type:
Neural Cells, PSC-Derived
Species:
Human
Sample Source:
Other
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Disease Modeling; Neuroscience

Scientific Resources

Educational Materials

(8)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

A H9 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 74.9% and 93.8% pure.

Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A H7 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 47.3% and 90.8% pure.

Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A 1C cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 18.9% and 86.7% pure.

Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.