Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
• No-wash removal of EasySep™ Releasable RapidSpheres™
- EasySep™ Release Human CD4 Positive Selection Kit (Catalog #17752)
- EasySep™ Release Human CD4 Positive Selection Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™ 50201, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Starting with a single-cell suspension of human PBMCs, the CD4+ T cell content of the isolated fraction is typically 96.1 ± 4.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 24.0% and 98.1%, respectively.