EasySep™ Human Myeloid DC Enrichment Kit
Immunomagnetic negative selection cell isolation kit

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Overview
Data Figures

Figure 1. EasySep™ Human Myeloid DC Enrichment Kit
Starting with 0.6 - 1.8% mDCs in fresh peripheral blood nucleated cells, the mDC content of the enriched fraction typically ranges from 50 - 90%* purity based on the mDC phenotype of Lineage (CD3, CD14, CD19, CD20, CD34, CD56)-negative, HLA DR-positive, and CD11c-positive. In the above example, the purities of the start and final enriched fractions are 1.1% and 80.7%, respectively. *If the mDC content of the starting sample is < 1.25%, the mDC content of the enriched fraction may be < 80%.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (6)
Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Publications (2)
Abstract
Abstract
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.