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Anti-Human CD20 Antibody, Clone 2H7

Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20

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Anti-Human CD20 Antibody, Clone 2H7

Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20

From: 140 USD
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Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20
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Overview

Antibody 2H7 reacts with CD20, an ~35 kDa non-glycosylated type 1 transmembrane protein in the MS4A protein family. The CD20 polypeptide transverses the cell membrane four times, with only a minor portion of the protein displayed on the cell surface. The epitope recognized by antibody 2H7 has been mapped to the amino acid sequence YNCEPANPSEKNSPST, located in the large extracellular loop of CD20. CD20 is expressed on pre-B cells, resting and activated B cells, some follicular dendritic cells, and a subset of T cells. Expression by B cells is lost upon their differentiation into plasma cells. By associating with several proteins, including the B cell receptor (CD79), MHC class I and II, CD53, CD81, and CD82, CD20 is involved in initiating intracellular signaling pathways that modulate the activation, proliferation, and differentiation of B cells. It is thought that CD20 forms multi-subunit ion channels that regulate calcium ion flux across the plasma membrane. Activation of CD20 is accompanied by pronounced phosphorylation of the cytoplasmic domain of the ~33 kDa apo-protein, with the appearance of 35 - 37 kDa isoforms that associate with Src family kinases such as Fyn, Lck and Lyn.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human Whole Blood CD20 Positive Selection Kit (Catalog #18685), EasySep™ Human CD19 Positive Selection Kit II (Catalog #17854), and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA).
Subtype
Primary Antibodies
Target Antigen
CD20
Alternative Names
B-lymphocyte antigen, B1, Bp35, Leu-16, MS4A1
Reactive Species
Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Human, Nancy Ma's Night Monkey, Pigtailed Macaque, Rhesus, Squirrel Monkey
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
B Cells
Species
Human, Non-Human Primate
Application
Cell Isolation, CyTOF, ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Area of Interest
Chimerism, HLA, Immunology, Transplantation Research
Clone
2H7
Gene ID
931
Isotype
IgG2b, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488 and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD20 Antibody, Clone 2H7, APC (filled histogram) or a mouse IgG2b, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa APC isotype control antibody is shown (open histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, FITC (filled histogram) or a mouse IgG2b, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa FITC isotype control antibody is shown (open histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, PE and antihuman CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD20 Antibody, Clone 2H7, PE. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for Unconjugated

Figure 5. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for Biotin-Conjugated

Figure 6. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin (Catalog #60072BT), followed by SAV APC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5 (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (Catalog #60072PS; solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, FITC
Catalog #
60008FI, 60008FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, Biotin
Catalog #
60008BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7
Catalog #
60008
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, APC
Catalog #
60008AZ.1, 60008AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488
Catalog #
60008AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, PE
Catalog #
60008PE.1, 60008PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5
Catalog #
60008PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, FITC
Catalog #
60008FI, 60008FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, Biotin
Catalog #
60008BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7
Catalog #
60008
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, APC
Catalog #
60008AZ.1, 60008AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488
Catalog #
60008AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, PE
Catalog #
60008PE.1, 60008PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5
Catalog #
60008PS
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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