EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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From: 590 USD

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Immunomagnetic positive selection cell isolation kit
From: 590 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Whole Blood Lymphoid Positive Selection Kit is designed to isolate CD3+ and CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.

This product replaces the EasySep™ Human Whole Blood Lymphoid Positive Selection Kit (Catalog #18684) and EasySep™ HLA Whole Blood Lymphoid Positive Selection Kit (Catalog #18684HLA) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99.9% purity
• No columns required
Components:
EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873)
    • EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
RoboSep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Kit (Catalog #17873RF)
    • EasySep™ HLA Chimerism Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells; Lymphocytes; T Cells
Species:
Human
Sample Source:
Buffy Coat; Peripheral Blood; Whole Blood
Selection Method:
Positive
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(12)
Brochure
HLA Antibodies for Purity Assessment
Brochure
EasySep™ Cell Separation Technology
Brochure
Cell Separation Products for HLA Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
4:55
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Video
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Webinar
56:02
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
Chimerism Analysis
Cell Isolation
Characterization

Data and Publications

Data

Starting with human whole blood, the lymphoid cell content (CD3+CD19+) of the isolated fraction is typically 99.5 ± 0.2% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 16.2% and 98.5%, respectively.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.