STEMdiff™ Endothelial Expansion Medium Kit

Expand human pluripotent stem cell-derived endothelial cells

STEMdiff™ Endothelial Expansion Medium Kit

Expand human pluripotent stem cell-derived endothelial cells

STEMdiff™ Endothelial Expansion Medium Kit
1 Kit
319 USD
Catalog # 08007

FBS-free medium for the expansion of human pluripotent stem cell-derived endothelial cells

What's Included

  • STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007)
    • STEMdiff™ Endothelial Expansion Basal Medium, 120 mL
    • STEMdiff™ Endothelial Expansion 5X Supplement, 30 mL
Products for Your Protocol

Overview

STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007) includes the xeno-free endothelial expansion basal medium and supplement, and must be used in conjunction with Animal Component-Free Cell Attachment Substrate. STEMdiff™ Endothelial Expansion Medium is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.

NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.

CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
Contains
Cell Culture Media and Supplements
Subtype
Specialized Media
Cell Type
Endothelial Cells
Species
Human
Application
Cell Culture, Expansion
Brand
STEMdiff
Area of Interest
Angiogenic Cell Research, Disease Modeling, Endothelial Cell Biology
Formulation
Serum-Free

Data Figures

Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit

In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).

Figure 2. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium

STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
08007
Lot #
All
Language
English
Catalog #
08007
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
08007
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
08007
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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