ImmunoCult™-XF

cGMP, serum-free, and xeno-free medium for the expansion of human T cells

ImmunoCult™-XF

cGMP, serum-free, and xeno-free medium for the expansion of human T cells

From: 217 USD
Catalog #
(Select a product)
cGMP, serum-free, and xeno-free medium for the expansion of human T cells
Add to Wish List

Product Advantages


  • Confidently expand T cells for use in clinical applications by using medium produced under relevant cGMPs

  • Reduce variability by expanding cells in serum- and xeno-free culture conditions

  • Achieve robust T cell expansion with high viability

  • Obtain T cells able to produce cytokines upon restimulation

  • Activate T cells bead-free by combining with ImmunoCult™ Human T Cell Activators

Overview

Support the culture of human T cells and enhance your cell therapy development by using high-performance, cGMP-manufactured ImmunoCult™-XF medium. Designed for robust expansion for use in clinical applications without the need for serum or serum derivatives, this medium is also phenol red-free, xeno-free and has no added cytokines—providing complete flexibility for your workflows. T cells can be harvested after just 10 - 12 days of culture and are able to produce cytokines, including interferon-gamma (IFN-γ) and IL4, upon restimulation. This ready-to-use medium is produced according to current GMP guidelines, ensuring the highest quality and consistency for reproducible results.

ImmunoCult™-XF can be used individually or in combination with ImmunoCult™ Human CD3/CD28 T Cell Activator or ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator for a gentle, bead-free activation of T cells. For more information on the expansion of T cells with ImmunoCult™, please explore the Product Information Sheet.
Subtype
Specialized Media
Cell Type
T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Regulatory
Species
Human
Application
Expansion
Brand
ImmunoCult
Area of Interest
Cancer, Immunology, Infectious Diseases, Cell Therapy Development
Formulation Category
Serum-Free, Xeno-Free

Data Figures

Culturing T Cells with ImmunoCult™-XF Supports Equivalent or Faster Expansion Compared to Commercial Alternatives

Figure 1. ImmunoCult™-XF Supports Equivalent or Faster T Cell Expansion Compared to Commercial Alternatives

T cells were isolated from human leukopaks using EasySep™ Human T Cell Isolation Kit (Catalog #17951), stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Catalog #10970), and cultured in either ImmunoCult™-XF medium supplemented with human recombinant IL-2 (rhIL-2) or other commercially available media. All commercial alternatives were prepared according to their respective manufacturer recommendations and supplemented with human AB serum or a serum derivative. The cells were analyzed at different time points for fold expansion relative to initial cell seeding density. ImmunoCult™-XF supported equal or higher expansion of total T cells compared to all commercial alternatives tested. T cells cultured in ImmunoCult™-XF underwent an average of 13-, 106-, and 876-fold expansion on days 5, 7, and 10, respectively. Data shown are mean ± SEM (n = 3 - 7 donors).

Culturing T Cells with ImmunoCult™-XF Supports Equivalent or Greater Expansion Compared to Commercial Alternatives

Figure 2. ImmunoCult™-XF Supports Equivalent or Greater T Cell Expansion than Other Commercial Alternatives

T cells were isolated from human leukopaks using EasySep™ Human T Cell Isolation Kit, stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, and cultured in either ImmunoCult™-XF medium supplemented with rhIL-2 or commercially available media. All commercial alternatives were prepared according to their respective manufacturer recommendations and supplemented with human AB serum or a serum derivative. T cells were analyzed on day 10 for fold expansion relative to the initial cell seeding density and culture viability. Compared to all commercial alternatives tested, ImmunoCult™-XF showed similar or significantly higher expansion of total T cells (shown in bars) while supporting high viability (shown by data points) (> 95% viable on day 10). Data shown are mean + SEM (n = 3 - 7 donors). *p < 0.05, **p < 0.01 as determined by ANOVA using Dunnett's Multiple Comparison post-hoc test.

Expansion of T Cells with Immunocult™-XF Express Similar Proportions of Both CD4+ and CD8+ Markers

Figure 3. T Cells Expanded in ImmunoCult™-XF Maintain Similar Proportions of CD4+ and CD8+ Cells Following 10 Days of Culture Expansion

CD3+ T cells were isolated from human leukopaks using EasySep™ Human T Cell Isolation Kit, stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, and cultured in ImmunoCult™-XF medium supplemented with rhIL-2. T cells were harvested on days (A) 0 and (B) 10 and analyzed by flow cytometry for the proportions of viable cells expressing CD4 and CD8. Data shown are from one representative donor; similar results were observed for the other 3 donors tested.

Expansion of T Cells Cultured with Immunocult™-XF Were Analyzed for Intracellular Cytokines such as IFN-Ɣ, TNF-α, and IL-4

Figure 4. T Cells Expanded in ImmunoCult™-XF Express Intracellular IFN-Ɣ, TNF-α, and IL-4

T cells were isolated from human leukopaks using EasySep™ Human T Cell Isolation Kit, stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, and cultured in ImmunoCult™-XF medium supplemented with rhIL-2. On day 10, T cells were harvested and analyzed for intracellular IFN-Ɣ, TNF-α, and IL-4 after stimulation with PMA and ionomycin for 4 hours, with Brefeldin A added after 2 hours. (A - C) IFN-Ɣ, TNF-α, and IL-4 production in CD4+ cells after stimulation; (D and E) IFN-Ɣ and TNF-α production in CD8+ cells after stimulation. Data shown are from one representative donor; similar results were observed for the other 3 donors tested. Unstimulated controls had < 0.5% IFN-Ɣ and TNF-α expression, or < 5% IL-4 expression (data not shown).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
ImmunoCult™-XF
Catalog #
100-0956
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-XF
Catalog #
100-0956
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)