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Achieve robust activation and expansion of human T cells for use in clinical applications–without the use of magnetic beads, feeder cells, or antigens.
This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF—a high-performance T cell expansion medium manufactured under relevant cGMP regulations and guidelines. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, providing the required primary and co-stimulatory signals for T cell culture and activation.
ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator is designed for cell therapy clinical research applications, qualified for use as an ancillary material (AM) following the framework outlined in USP<1043> and/or PH. EUR. 5.2.12. STEMCELL can work with you to qualify this reagent as an AM under an approved Investigational New Drug (IND) application, Biological Licensing Application (BLA), or Clinical Trial Application (CTA). Learn more about how we can support your regulatory needs here.
Figure 1. Morphology of Activated Human T Cells Stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator
A clustered morphology is seen in activated human T cells. Cells were isolated using EasySep™ Human T Cell Isolation Kit, stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator for 3 days in ImmunoCult™-XF supplemented with recombinant human interleukin-2 (rhIL-2).
Figure 2. Activation of Human T Cells Stimulated with ImmunoCult™ Human CD3/CD28/CD2 Activator.
Human T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator and cultured in ImmunoCult™-XF. Activation of viable CD4+ and CD8+ T cells were assessed by CD25 expression, using flow cytometry. Following 3 days of culture, the frequency of CD25-positive cells was (A) 91.4% for CD4+ T cells and (B) 87.8% for CD8+ T cells. The gray line depicts day 3 CD4+ and CD8+ T cells cultured without ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator.
Figure 3. Robust Human T Cell Expansion and High Viability Achieved Using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator
Human T cells were expanded over 12 days with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator in ImmunoCult™-XF supplemented with rhIL-2. On day 0, 1 x 10^6 isolated human T cells were stimulated with 25 μL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator in ImmunoCult™-XF supplemented with rhIL-2.No additional ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator was added during the 12-day culture period (mean ± SD in 3 experiments with 7 donors).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
cGMP, serum-free, and xeno-free medium for the expansion of human T cells
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ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator
Quality Statement:
THIS PRODUCT IS MANUFACTURED AND TESTED FOLLOWING RELEVANT CGMPs UNDER A CERTIFIED QUALITY MANAGEMENT SYSTEM. PRODUCT IS FOR INVESTIGATIONAL OR RESEARCH USE. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.