Overview
This antibody clone has been verified for labeling neural stem and progenitor cells grown with NeuroCult™ Proliferation Kit (Mouse & Rat; Catalog #05702).
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Data for Unconjugated
(A) Embryonic mouse cortical tissue was cultured using the NeuroCult™ Proliferation Kit (Mouse), then fixed and labeled with Anti-Rat Nestin Antibody, Clone Rat401, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody (Anti-Dextran Antibody, Clone DX1, Catalog #60026) followed by goat anti-mouse IgG, FITC (without DAPI staining).
(B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit, then fixed and labeled with Anti-Rat Nestin Antibody, Clone Rat401, followed by goat anti-mouse IgG, FITC. Nestin expression is down-regulated during neuronal differentiation. Inset shows cells labeled with a positive control antibody (anti-neuronal class III beta-tubulin antibody) followed by goat anti-mouse IgG, FITC.
(C) Western blot analysis of denatured/reduced cell lysates with Anti-Rat Nestin Antibody, Clone Rat401. Lane 1, mouse neural progenitor cells cultured with the NeuroCult™ Proliferation Kit (Mouse), lane 2, HT1080 fibrosarcoma cells (negative control), lane 3, adult rat brain cells (negative control).