Anti-Rat Nestin Antibody, Clone Rat401

Mouse monoclonal IgG1 antibody against mouse, rat nestin

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Mouse monoclonal IgG1 antibody against mouse, rat nestin
304 USD

Overview

The Rat401 antibody reacts with mouse and rat nestin, a > 170 kDa Class VI intermediate filament (IF) protein expressed in neuroepithelial stem and progenitor cells and some other cell types, including pancreatic islet progenitor cells, angiogenic endothelial cells, glioma cells, and bone marrow mesenchymal stem cells. Expression is down-regulated following neural differentiation, and within the adult nervous system is limited mainly to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin assembles into heterodimers with vimentin or α-internexin by a phosphorylation-dependent process to form IFs and is thus involved in structural organization of the cell. It is required for the survival, renewal, and proliferation of neural progenitor cells and may also be involved in growth cone guidance during neuronal differentiation. During differentiation, nestin-containing filaments are replaced by cell type-specific IFs such as GFAP.

This antibody clone has been verified for labeling neural stem and progenitor cells grown with NeuroCult™ Proliferation Kit (Mouse & Rat; Catalog #05702).
Subtype:
Primary Antibodies
Target Antigen:
Nestin
Alternative Names:
NES, Type VI intermediate filament (IF) protein
Reactive Species:
Mouse; Rat
Conjugation:
Unconjugated
Host Species:
Mouse
Cell Type:
Neurons
Application:
Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Western Blotting
Area of Interest:
Neuroscience
Clone:
Rat401
Gene ID:
18008
Isotype:
IgG1, kappa

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Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Embryonic mouse cortical tissue was cultured using the NeuroCult™ Proliferation Kit (Mouse), then fixed and labeled with Anti-Rat Nestin Antibody, Clone Rat401, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody (Anti-Dextran Antibody, Clone DX1, Catalog #60026) followed by goat anti-mouse IgG, FITC (without DAPI staining).
(B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit, then fixed and labeled with Anti-Rat Nestin Antibody, Clone Rat401, followed by goat anti-mouse IgG, FITC. Nestin expression is down-regulated during neuronal differentiation. Inset shows cells labeled with a positive control antibody (anti-neuronal class III beta-tubulin antibody) followed by goat anti-mouse IgG, FITC.
(C) Western blot analysis of denatured/reduced cell lysates with Anti-Rat Nestin Antibody, Clone Rat401. Lane 1, mouse neural progenitor cells cultured with the NeuroCult™ Proliferation Kit (Mouse), lane 2, HT1080 fibrosarcoma cells (negative control), lane 3, adult rat brain cells (negative control).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.