PBS-MINI MagDrive Bioreactor

Compact bioreactor with single-use vessels for high-throughput, 3D suspension culture of hPSCs and other cell types
Catalog #
100-1005_C
Compact bioreactor with single-use vessels for high-throughput, 3D suspension culture of hPSCs and other cell types
From: 975 USD

Overview

Reliably and rapidly scale up your 3D cell cultures and suspensions with the PBS-MINI MagDrive Bioreactor Base Unit and MAG Single-Use Vessels. The gentle yet efficient mixing provided by the Vertical-Wheel™ impeller enables the expansion of shear-sensitive cells without anti-foaming agents or shear protectants. Ideal for human pluripotent stem cells (hPSCs), the compact, sealed Base Unit can be used inside incubators. Conveniently control your culture system with a speed dial and digital display, and visualize cells in low-light conditions using built-in LED lights.

PBS-MINI 0.1 L and 0.5 L MAG Single-Use Vessels are sold separately in packs of 4. These disposable vessels are animal component-free and meet the requirements for USP Class VI Testing for Plastics <88> and ISO 10993.

The PBS-MINI MagDrive Bioreactor Base Unit includes a power adapter that is compatible with Type A electrical outlets and 120V or 240V A/C inputs. A separate wall adaptor is needed for use outside North America. An 18-month warranty is provided by PBS Biotech Inc., valid from the purchase date. This product is for Research Use Only.

For more information, contact techsupport@stemcell.com.
Advantages
⦁ COMPACT. The compact, stainless steel Base Unit fits where you need it, even allowing operation inside incubators.
⦁ SAFE & EFFICIENT EXPANSION. The Vertical-Wheel™ impeller and U-shaped base create a low-shear mixing environment that is optimal for culturing hPSC aggregates of uniform size.
⦁ EASY AGGREGATE RECOVERY. The U-shaped base collects aggregates and the wide diameter cap provides easy access for aggregate recovery.
⦁ A PERFECT PAIR. The Base Unit is designed to be used with the 0.1 L and 0.5 L PBS-MINI MAG Single-Use Vessels, magnetically driving the vessels via MagDrive technology.
Application
Cell Culture, Expansion
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
PBS-MINI MagDrive Bioreactor Base Unit
Catalog #
100-1005
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
PBS-MINI 0.1 MAG Single-Use Vessel
Catalog #
100-1006
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
PBS-MINI 0.5 MAG Single-Use Vessel
Catalog #
100-1007
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
PBS-MINI MagDrive Bioreactor
Catalog #
100-1005
Lot #
All
Language
English

Educational Materials (7)

Brochure
Products for Human Pluripotent Stem Cells
Technical Bulletin
mTeSR™3D: Clump Passaging Method for Suspension Culture hPSCs
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Video
How to Count 3D Sphere Cultures (Mammospheres, Neurospheres, Cancer Spheroids, and PSC Aggregates)
2:05
How to Count 3D Sphere Cultures (Mammospheres, Neurospheres, Cancer Spheroids, and PSC Aggregates)
Video
Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium
1:58
Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium
Video
How to Passage hPSC Aggregates in mTeSR™3D Suspension Culture with a Clump Passaging Method
5:14
How to Passage hPSC Aggregates in mTeSR™3D Suspension Culture with a Clump Passaging Method
Scientific Poster
Optimized Media and Workflow for the Expansion of Human Pluripotent Stem Cells as Aggregates in Suspension Cultures

Data and Publications

Data

Figure 1. Consistently High Daily Fold Expansion of Human Pluripotent Stem Cells (hPSCs) Is Achieved with the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels

Daily fold expansions of a human embryonic stem (ES) cell line (H9) and human induced pluripotent stem (iPS) cell line (WLS-1C), cultured in PBS-MINI (A) 0.1 and (B) 0.5 MAG Single-Use Vessels, show consistently high growth. The cells were seeded at a density of 8 x 104 viable cells/mL in 100 mL (for PBS-MINI 0.1 MAG) and 3 - 8 x 104 viable cells/mL in 500 mL (for PBS-MINI 0.5 MAG), and cultured for 4 days using mTeSR™3D (Catalog #03950). Fold expansion of each cell line was comparable to their growth in Nalgene™ filter bottles (Control), when seeded at a density of 1 x 105 viable cells/mL in 30 mL. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).

Figure 2. Culturing hPSCs in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels Achieves High Cell Viability

End-of-passage viabilities of a human ES cell line (H9) and human iPS cell line (WLS-1C) cultured in mTeSR™3D show that the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels support expansion of hPSCs at (A) 100 mL (with PBS-MINI 0.1 MAG) and (B) 500 mL (with PBS-MINI 0.5 MAG) culture volumes. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).

Figure 3. Markers of Undifferentiated hPSCs Harvested from the PBS-MINI 0.5 MAG Single-Use Vessels

1C and H9 hPSC lines after 9 passages in the PBS-MINI 0.5 MAG Single-Use Vessels using mTeSR™3D show high OCT-4 and TRA-1-60 expression. Error bars are +/- SD (n = 1).

Figure 4. Morphology of hPSC Aggregates Is Optimal When Using the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels

hPSCs, expanded in suspension using mTeSR™3D in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels, exhibit the typical morphological characteristics of hPSC aggregates in suspension culture, including a mostly spherical shape, clear edges (not smooth or shiny), an even color, and a dimpled or pock-marked appearance. Aggregates are typically 350-400 µm in size. Scale bar = 500 µm.

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