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Human Umbilical Vein Endothelial Cells

Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.

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Human Umbilical Vein Endothelial Cells

Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.

From: 425 USD
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Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.
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Product Advantages


  • Obtain over 500,000 HUVECs from a single vial—guaranteed— isolated from a healthy donor

  • Achieve over 16 population doublings of HUVECs when cultured in xeno-free EC-Cult™-XF medium

  • Obtain HUVECs frozen at early passage 2, with over 90% viability prior to cryopreservation

  • Retain high expression of endothelial markers CD31 and CD144 through later passages with EC-Cult™-XF medium

  • Observe functional properties such as the formation of tubular networks and acetylated LDL uptake

What's Included

  • Frozen Human Umbilical Vein Endothelial Cells
    • Products for Your Protocol
    To see all required products for your protocol, please consult the Protocols and Documentation.
    1. EC-Cult™-XF Culture Kit
      EC-Cult™-XF Culture Kit

      Culture kit for derivation and proliferation of human endothelial colony-forming cells (ECFCs) and mature endothelial cells

    2. ThawSTAR® CFT2 Automated Thawing System
      ThawSTAR® CFT2 Automated Thawing System

      Automated cell thawing system for consistent thawing performance

    Overview

    Streamline your assays with ready-to-use, ethically sourced, human umbilical vein endothelial cells (HUVECs). These cells are isolated from healthy single-donor umbilical cord veins (using non-invasive collagenase digestion) into EC-Cult™-XF medium and cryopreserved at passage 2 in animal component-free, cGMP-manufactured CryoStor® CS10 medium. Obtain a large number of HUVECs from a single vial for consistent results.

    HUVECs are collected using Institutional Review Board (IRB)-approved consent forms and protocols. They can show expression of endothelial markers through later passages, an ability to form tubular networks, as well as uptake of acetylated LDL. HUVECs can be used as an endothelial cell model to study physiological and pathological pathways, such as inflammation, angiogenesis, oxidative stress, hypoxia, and blood vessel formation. With personalized service, custom products, and flexible delivery times, we help you get the cells you need.

    Additional documentation and high-resolution HLA typing (Class I and Class II alleles) and CMV status is available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Full product information is provided on the Certificate of Analysis.

    Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

    Browse our Frequently Asked Questions (FAQs) on Primary Cells.
    Contains
    CryoStor® CS10
    Cell Type
    HUVECs
    Species
    Human
    Cell and Tissue Source
    Other
    Area of Interest
    Endothelial Cell Biology
    Donor Status
    Normal
    Purity
    ≥ 95% CD31+CD144+ and ≤ 5% CD45+ by flow cytometry

    Data Figures

    Phase contrast image of HUVECs cultured in EC-Cult™-XF shows how these cells grow as monolayers and retain their cobblestone-like morphology of endothelial cells.

    Figure 1. HUVECs Cultured in EC-Cult™-XF Have Cobblestone-Like Morphology

    Phase-contrast image of HUVECs cultured in EC-Cult™-XF. Cells grow as monolayers and retain their typical cobblestone-like morphology of endothelial cells.

    Flow cytometry analysis shows that HUVECs cultured in EC-Cult™-XF medium retain the endothelial markers CD31 and CD144 through 19 passages better than those cultured in a commercial medium.

    Figure 2. HUVECs Cultured Long-Term in Complete EC-Cult™-XF Retain Purity of Endothelial Markers.

    HUVECs cultured and passaged in complete EC-Cult™-XF medium were compared to HUVECs cultured and passaged in an alternative commercial medium. Both sets of HUVECs were then analyzed by flow cytometry. Cells cultured in EC-Cult™-XF medium had higher retention of the endothelial markers CD31 and CD144 through 19 passages.

    HUVECs can expand for higher passage numbers when cultured in EC-Cult™-XF medium when compared to alternative commercial media.

    Figure 3. HUVECs Can Be Expanded For Greater Passage Numbers in EC-Cult™-XF Than in Alternative Commercial Media

    HUVECs were cultured and expanded in EC-Cult™-XF medium or alternative commercial medium (A and B). HUVECs expanded in complete EC-Cult™-XF medium outperformed those in the alternative media, with population doublings continuing to increase beyond passage 16.

    HUVECs, from passages 2 and 7, were plated in EC-Cult™-XF medium and grown for 24 hours. Cells were imaged at 2, 6, 18, and 24 hour time points, showing the ability to form defined tubular branching points as early as after 2 hours of plating.

    Figure 4. HUVECs Form Tubular Networks When Cultured in Complete EC-Cult™-XF Medium

    HUVECs, from passage 2 and passage 7, were plated at 10,000 cells/well (96-well plate) in complete EC-Cult™-XF medium and grown for 24 hours on Corning® Matrigel®. Cells were imaged at different time points and shown to form defined tubular branching points as early as after 2 hours of plating.

    Labeled acetylated LDL was added to HUVECs at early (P2) and late (P7) passages. Cells were then imaged using a confocal microscope and shown to be functionally active and able to incorporate acetylated LDL at both P2 and P7.

    Figure 5. HUVECs are Functionally Active at Early and Late Passages

    The ability of HUVECs to metabolise acetylated LDL was examined by adding labeled acetylated LDL into the cells and incubated for 4-6 hours before imaging using a confocal microscope. Cells were shown to be functionally active and able to incorporate acetylated LDL at early (P2) and late (P7) passage when cultured in EC-Cult™-XF Medium.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Document Type
    Product Information Sheet
    Product Name
    Human Umbilical Vein Endothelial Cells (HUVECs)
    Catalog #
    200-0630
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages

    Resources and Publications

    Educational Materials (4)

    Human Primary Cells
    Brochure
    Human Primary Cells
    Optimized Endothelial Cell Culture Workflow
    Brochure
    Optimized Endothelial Cell Culture Workflow
    How to Thaw Frozen Human Primary Cells
    6:05
    Video
    How to Thaw Frozen Human Primary Cells
    Endothelial Cells, Angiogenesis, and Vasculogenesis
    Mini Review
    Endothelial Cells, Angiogenesis, and Vasculogenesis

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    Quality Statement:

    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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