Human Recombinant IL-3 (CHO-expressed)

Interleukin 3

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Interleukin 3
From: 186 USD

Overview

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).
Alternative Names:
Hematopoietic growth factor, Interleukin-3, Mast cell growth factor, MCGF, MGC79398, MGC79399, Multi-CSF, Multilineage-colony-stimulating factor, Multiple colony-stimulating factor, Multipotential colony-stimulating factor, P-cell-stimulating factor
Cell Type:
T Cells; Dendritic Cells; Granulocytes and Subsets; Macrophages; NK Cells; Hematopoietic Stem and Progenitor Cells
Species:
Human
Area of Interest:
Immunology
Molecular Weight:
15.1 kDa
Purity:
≥ 95%

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Data and Publications

Data

(A) The biological activity of Human Recombinant IL-3 (CHO-expressed) was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 0.1 ng/mL. (B) 2 μg of Human Recombinant IL-3 (CHO-expressed) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant IL-3 (CHO-expressed) has a predicted molecular mass of 15.1 kDa.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.