Human Recombinant EPO

Erythropoietin
Catalog #
78007_C
Erythropoietin
From: 76 USD

Overview

Erythropoietin (EPO) is a glycoprotein growth factor that is produced primarily in the kidney in response to hypoxia or anemia. It is the principal physiological regulator of erythropoiesis. EPO promotes erythropoiesis by binding to a homodimeric cell surface receptor that activates JAK2/STAT5, PI3K/AKT, and MAPK pathways, and stimulates the proliferation and differentiation of erythroid progenitor cells (Jelkmann; Kuhrt & Wojchowski).
Subtype
Cytokines
Alternative Names
Epoetin, Erythropoietin, EP
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology
Molecular Weight
18.4 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant EPO
Catalog #
78007, 78007.1, 78007.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant EPO
Catalog #
78007, 78007.1, 78007.2
Lot #
All
Language
English

Educational Materials (8)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Reliable Antibodies for Your Research
Brochure
High Affinity Anti-EPO Reagents for Consistent Results
Wallchart
Small Molecules, Big Impact in Cancer Research
Wallchart
Human Hematopoietic Stem and Progenitor Cell Phenotyping
Webinar
A Guide to Erythropoiesis - EPO Signaling and Erythroid Development
40:56
A Guide to Erythropoiesis - EPO Signaling and Erythroid Development
Webinar
Serum- and Feeder-Free Differentiation of Erythroid Progenitor Cells from hPSCs
31:39
Serum- and Feeder-Free Differentiation of Erythroid Progenitor Cells from hPSCs
Mini Review
Erythropoietin (EPO): Properties, Biological Functions, and Measurement

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant EPO was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which the cell proliferation is at 50% of maximum. The EC50 in the above example is less than 0.6 ng/mL. (B) Human Recombinant EPO stimulates the proliferation and differentiation of erythroid progenitor cells, and was validated by titration in a CFU assay on human bone marrow MNCs, using MethoCult™ SF H4236 (Catalog #04236). The total number of colonies at each EPO concentration was enumerated using STEMvision™ (Catalog #22006). (C) 5 μg of Human Recombinant EPO was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant EPO polypeptide has a predicted molecular mass of 18.4 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26 - 36 kDa in SDS-PAGE.

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