Human Recombinant BMP-2

Bone morphogenetic protein 2

Human Recombinant BMP-2

Bone morphogenetic protein 2

From: 465 USD
Catalog #
78004_C
Bone morphogenetic protein 2

Overview

Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor beta (TGF-β) superfamily. BMP-2 is a disulfide-linked homodimer, acts as a ligand for complexes of type I and II BMP receptors, and primarily activates SMAD1/5/8 signaling (Nohe et al.). BMP-2 is a potent differentiation factor and directs human pluripotent stem cells (hPSCs) towards various cell types including extra-embryonic endoderm, mesenchymal cells, and chondrocytes (Pera et al.). Although BMP-2 expression is low in healthy cartilage, its expression is upregulated at the site of cartilage damage (Blaney Davidson et al.). BMP-2 induces bone and cartilage formation in vitro and is able to induce chondrogenesis in human mesenchymal stem cells (Schmitt et al.).
Subtype
Cytokines
Alternative Names
BDA2, BMP-2A, BMP2, Bone morphogenetic protein 2A
Cell Type
Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Neuroscience, Stem Cell Biology
Molecular Weight
26 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant BMP-2 was tested by its ability to promote alkaline phosphatase production of ATDC-5 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the above example is 0.09 μg/mL.
(B) 2 μg of Human Recombinant BMP-2 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant BMP-2 has a predicted molecular mass of 26 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78004, 78004.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78004, 78004.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

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