Human Mesenchymal Stem Cell qPCR Array

For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages

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Human Mesenchymal Stem Cell qPCR Array

For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages

From: 230 USD
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For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages
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What's Included

  • Human Mesenchymal Stem Cell qPCR Array, Type 1 (Catalog #07541)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Mesenchymal Stem Cell qPCR Array, Type 3 (Catalog #07543)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 96-well plate
    • TBP qPCR Control Template, 1 vial
  • Human Mesenchymal Stem Cell qPCR Array, Type 5 (Catalog #07545)
    • Human Mesenchymal Stem Cell qPCR Array, 1 x 384-well plate
    • TBP qPCR Control Template, 1 vial
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The Human Mesenchymal Stem Cell Quantitative Polymerase Chain Reaction (qPCR) Array is designed for characterization of human mesenchymal stem and progenitor cells (MSCs) and their differentiated chondrogenic, adipogenic, or osteogenic progeny. MSCs are self-renewing, multipotent precursors found in the adherent fraction of bone marrow and stromal compartments in multiple tissues. MSCs can be expanded in vitro to generate mesenchymal stromal cell cultures, which, under appropriate conditions, can differentiate into adipocytes, chondrocytes, and osteoblasts. This qPCR array provides the gene expression profile of MSCs and their trilineage derivatives following in vitro differentiation. Genes were selected based on their demonstrated differential expression in MSCs (Silva et al.) or in MSC-derived chondrogenic (Herlofsen et al.; Yoo et al.), adipogenic (Hung et al.; Menssen et al.), or osteogenic lineage cells (Kulterer et al.).

qPCR is used to determine changes in steady-state mRNA levels of gene expression across multiple samples, generally normalized to the relative expression of internal control genes. Gene-specific primers amplify target sequences within cDNA pools reverse-transcribed from mRNA and, as with TaqMan® technology, hybridized sequence-specific probes provide a fluorescent signal from the 5' exonuclease activity of Taq DNA polymerase. The accumulation rate of the fluorescent signal is used to quantify the sample cDNA, and thereby the amount of mRNA in the original cell lysate.

This qPCR array contains validated primers and probes for detection of 90 genes whose expression is correlated with MSCs or their differentiated derivatives, as well as six endogenous (housekeeping) control genes. TBP (TATA box-binding protein) qPCR Control Template is provided as a synthetic DNA positive control. Additional gene information and a qPCR analysis tool are available at www.stemcell.com/qPCRanalysis.
Cell Type
Adipocytes, Chondrocytes, Mesenchymal Stem and Progenitor Cells, Osteoblasts
Species
Human
Application
Genome Editing
Area of Interest
Stem Cell Biology

Data Figures

Figure 1. Human Mesenchymal Stem Cell qPCR Array Plate Configuration

This illustration shows a quadrant of 96 unique primer-probe assays available on a 384-well qPCR plate (A). There are four identical quadrants in total, which together form a complete array of 384 assays used in the Human Mesenchymal Stem Cell Array plate configuration. The diagram also indicates the wells containing the TBP qPCR control cocktail (H12, H24, P12, P24), which serve as reference wells for assay normalization (B).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
07541, 07543, 07545
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
07541, 07543, 07545
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
07541
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
07543
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
07545
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more