EasySep™ Mouse ILC2 Enrichment Kit

Immunomagnetic negative selection kit

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EasySep™ Mouse ILC2 Enrichment Kit

Immunomagnetic negative selection kit

From: 849 USD
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Immunomagnetic negative selection kit
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Product Advantages


  • Fast, easy-to-use and column-free

  • Isolated cells are untouched

  • Facilitates rapid flow sorting of ILC2s

What's Included

  • EasySep™ Mouse ILC2 Enrichment Kit (Catalog #19842)
    • EasySep™ Mouse Enrichment Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL

Overview

This product is designed to enrich ILC2's (Group 2 Innate Lymphoid Cells) from single-cell suspensions of lung tissue or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-ILC2 cells and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns.
Magnet Compatibility
EasySep™ Magnet (Catalog #18000)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Mouse
Sample Source
Other
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Starting with a naïve mouse lung single-cell suspension, the ILC2 content (CD45+Lin-CD278+CD90.2+ST2+) of the final enriched fraction typically ranges from 2.2 - 7.1%. In the above example, the percentage of ILC2s in the start and final enriched fractions are 0.8% and 6.5% (or 0.9% and 22.3% of CD45+ cells), respectively. NOTE: The ILC2 content of the start fraction typically ranges from 0.1 - 1%.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19842
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19842
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19842
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
19842
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more