EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Immunomagnetic positive selection kit using particle release technology
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EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit -
Label for EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer
Overview
The EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit is designed to isolate CD271⁺ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.
Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.
Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages:
⦁ Highly purified human PSC-derived neural crest cells isolated in less than 20 minutes
⦁ No-wash removal of EasySep™ Releasable RapidSpheres™
⦁ Compatible with downstream differentiation protocols
⦁ No-wash removal of EasySep™ Releasable RapidSpheres™
⦁ Compatible with downstream differentiation protocols
Components:
- EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Cocktail, 2 x 1.0 mL
- EasySep™ Isolation Cocktail Enhancer, 1.0 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1.0 mL
- EasySep™ Releasable RapidSpheres™ 50201, 2 x 1.0 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
Subtype:
Cell Isolation Kits
Cell Type:
Neural Cells, PSC-Derived
Species:
Human
Sample Source:
Other
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Disease Modeling; Neuroscience
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STEMdiff™ Neural Crest Differentiation Kit
Cell culture kit for establishment of hPSC-derived neural crest cells -
Serum-free neurophysiological basal medium for improved neuronal function -
MesenCult™-ACF Chondrogenic Differentiation Kit
Animal component-free medium for the differentiation of MSCs into chondrocytes -
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Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
100-0047
All
100-0047
All
100-0047
All
100-0047
All
Educational Materials
(8)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry
Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.