EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

1 x 10^9 cells
Catalog # 100-0047
752 USD
Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. RoboSep™ Buffer 2
    RoboSep™ Buffer 2

    Cell separation buffer

Overview

The EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit is designed to isolate CD271⁺ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.

Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages
⦁ Highly purified human PSC-derived neural crest cells isolated in less than 20 minutes
⦁ No-wash removal of EasySep™ Releasable RapidSpheres™
⦁ Compatible with downstream differentiation protocols
Components
  • EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Cocktail, 2 x 1.0 mL
  • EasySep™ Isolation Cocktail Enhancer, 1.0 mL
  • EasySep™ Release Buffer (Concentrate), 3 x 1.0 mL
  • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1.0 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
Subtype
Cell Isolation Kits
Cell Type
Neural Cells, PSC-Derived
Species
Human
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Disease Modeling, Neuroscience

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Catalog #
100-0047
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit
Catalog #
100-0047
Lot #
All
Language
English

Educational Materials (8)

Brochure
hPSC-Derived Neural Cell Research
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)
2:12
How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen)
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Webinar
hPSC-Derived Neural Crest: Induction and Axial Patterning
54:21
hPSC-Derived Neural Crest: Induction and Axial Patterning
Scientific Poster
Efficient Generation of Human Pluripotent Stem Cells to Neural Crest Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

A H9 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 74.9% and 93.8% pure.

Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A H7 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 47.3% and 90.8% pure.

Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A 1C cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 18.9% and 86.7% pure.

Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

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