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EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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From: 546 USD

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Immunomagnetic positive selection cell isolation kit
From: 546 USD

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Overview

The EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit is designed to isolate highly purified CD25+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. The kit targets CD25 regulatory T cells for positive selection with a PE-labeled antibody directed against the CD25 surface marker. Desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube.
Advantages:
• Fast and easy-to-use
• Up to 93% purity
• No columns required
Components:
  • EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782)
    • EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
    • EasySep™ PE Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • EasySep™ Mouse FcR Blocker, 0.5 mL
  • RoboSep™ Mouse CD25 Regulatory T cell Positive Selection Kit (Catalog #18782RF)
    • EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail, 0.5 mL
    • EasySep™ PE Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • EasySep™ Mouse FcR Blocker, 0.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, Regulatory; T Cells, CD4+
Species:
Mouse
Sample Source:
Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(11)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Profile

Figure 1. Typical EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Profile

Starting with mouse splenocytes, the CD25 regulatory T cell content (CD4+CD25+FOXP3+) of the isolated fraction typically ranges from 80 - 93%. In the example above, the final purities of the start and isolated fractions are 2.1% and 87.5%, respectively.

Publications

(1)
Immunity 2016 MAY

Thymus-Derived Regulatory T Cells Are Positively Selected on Natural Self-Antigen through Cognate Interactions of High Functional Avidity.

Kieback E et al.

Abstract

Regulatory T (Treg) cells expressing Foxp3 transcripton factor are essential for immune homeostasis. They arise in the thymus as a separate lineage from conventional CD4(+)Foxp3(-) T (Tconv) cells. Here, we show that the thymic development of Treg cells depends on the expression of their endogenous cognate self-antigen. The formation of these cells was impaired in mice lacking this self-antigen, while Tconv cell development was not negatively affected. Thymus-derived Treg cells were selected by self-antigens in a specific manner, while autoreactive Tconv cells were produced through degenerate recognition of distinct antigens. These distinct modes of development were associated with the expression of T cell receptor of higher functional avidity for self-antigen by Treg cells than Tconv cells, a difference subsequently essential for the control of autoimmunity. Our study documents how self-antigens define the repertoire of thymus-derived Treg cells to subsequently endow this cell type with the capacity to undermine autoimmune attack.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.