EasySep™ Mouse CD11c Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

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EasySep™ Mouse CD11c Positive Selection Kit II

Immunomagnetic positive selection of mouse CD11c+ cells (e.g. dendritic cells (DC)) from mouse splenocytes or other tissues

2 x 109 cells
Catalog #18780
688 USD

RoboSep™ Mouse CD11c Positive Selection Kit II

EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips

2 x 109 cells
Catalog #18780RF
745 USD

EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium

Immunomagnetic positive selection of CD11c+ cells (e.g. dendritic cells (DC)) from mouse splenocytes or other tissues

2 x 109 cells
Catalog #18781
694 USD

RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium

EasySep™ kit with RoboSep™ buffer, RoboSep™ filter tips and Spleen Dissociation Media

2 x 109 cells
Catalog #18781RF
715 USD

Required Products

Overview

The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

This product replaces the EasySep™ Mouse CD11c Positive Selection Kit (Catalog #18758) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Advantages:
• Fast and easy-to-use
• Up to 95% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components:
  • EasySep™ Mouse CD11c Positive Selection Kit II (Catalog #18780)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
  • RoboSep™ Mouse CD11c Positive Selection Kit II (Catalog #18780RF)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
  • EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium (Catalog #18781)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
    • Spleen Dissociation Medium (Catalog #07915)
  • RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium (Catalog #18781RF)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
    • Spleen Dissociation Medium (Catalog #07915)
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Dendritic Cells; Granulocytes and Subsets; Monocytes; NK Cells
Species:
Mouse
Sample Source:
Bone Marrow; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

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Educational Materials

(7)

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical EasySep™ CD11c Positive Selection Profile

Figure 1. Typical EasySep™ CD11c Positive Selection Profile

Starting with mouse splenocytes, the CD11c+ cell content of the enriched fraction is typically 86.8 ± 9.7% (gated on viable singlet cells, mean ± SD using the purple EasySep™ Magnet). In the example above, the final purities of the start and isolated fraction are 5.7% and 92.3%, respectively.

Publications

(2)
Immunity 2017 AUG

Dendritic Cells but Not Macrophages Sense Tumor Mitochondrial DNA for Cross-priming through Signal Regulatory Protein α Signaling.

Xu MM et al.

Abstract

Inhibition of cytosolic DNA sensing represents a strategy that tumor cells use for immune evasion, but the underlying mechanisms are unclear. Here we have shown that CD47-signal regulatory protein α (SIRPα) axis dictates the fate of ingested DNA in DCs for immune evasion. Although macrophages were more potent in uptaking tumor DNA, increase of DNA sensing by blocking the interaction of SIRPα with CD47 preferentially occurred in dendritic cells (DCs) but not in macrophages. Mechanistically, CD47 blockade enabled the activation of NADPH oxidase NOX2 in DCs, which in turn inhibited phagosomal acidification and reduced the degradation of tumor mitochondrial DNA (mtDNA) in DCs. mtDNA was recognized by cyclic-GMP-AMP synthase (cGAS) in the DC cytosol, contributing to type I interferon (IFN) production and antitumor adaptive immunity. Thus, our findings have demonstrated how tumor cells inhibit innate sensing in DCs and suggested that the CD47-SIRPα axis is critical for DC-driven antitumor immunity.
Journal of Immunology 2016 MAY

LSm14A Plays a Critical Role in Antiviral Immune Responses by Regulating MITA Level in a Cell-Specific Manner.

Liu T-T et al.

Abstract

Viral infection triggers induction of antiviral cytokines and effectors, which are critical mediators of innate antiviral immune response. It has been shown that the processing body-associated protein LSm14A is involved in the induction of antiviral cytokines in cell lines but in vivo evidence is lacking. By generating LSm14A-deficient mice, in this study, we show that LSm14A plays a critical and specific role in the induction of antiviral cytokines in dendritic cells (DCs) but not in macrophages and fibroblasts. Induction of antiviral cytokines triggered by the DNA viruses HSV-1 and murid herpesvirus 68 and the RNA virus vesicular stomatitis virus but not Sendai virus was impaired in Lsm14a(-/-) DCs, which is correlated to the functions of the adaptor protein MITA/STING in the antiviral signaling pathways. LSm14A deficiency specifically downregulated MITA/STING level in DCs by impairing its nuclear mRNA precursor processing and subsequently impaired antiviral innate and adaptive immune responses. Our findings reveal a nuclear mRNA precursor processing and cell-specific regulatory mechanism of antiviral immune responses.
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