EasySep™ Human Whole Blood CD34 Positive Selection Kit II
Immunomagnetic positive selection kit
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Required Products
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EasySep™ BufferCell separation buffer
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"The Big Easy" EasySep™ MagnetMagnet for column-free immunomagnetic separation
Overview
The EasySep™ Human Whole Blood CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.
This product can be used instead of the EasySep™ Human Whole Blood CD34 Positive Selection Kit (Catalog #18086).
If isolating CD34+ cells from fresh cord blood, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.
This product can be used instead of the EasySep™ Human Whole Blood CD34 Positive Selection Kit (Catalog #18086).
If isolating CD34+ cells from fresh cord blood, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.
Advantages
• Fast and easy-to-use
• No columns required
• No columns required
Components
- EasySep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879)
- EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Human Whole Blood CD34 Positive Selection Kit II (Catalog #17879RF)
- EasySep™ Human Whole Blood CD34 Positive Selection Cocktail II, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology, Stem Cell Biology
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
EasySep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
RoboSep™ Human Whole Blood CD34 Positive Selection Kit II
|
Catalog #
17879RF |
Lot #
All |
Language
English |
Educational Materials (6)
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Human Immune Cytokines
Video
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for
Chimerism Analysis
Data and Publications
Data
Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using RoboSep™-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
