EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
Immunomagnetic negative selection cell isolation kit
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Required Products
-
EasySep™ MagnetMagnet for column-free immunomagnetic separation
-
EasySep™ BufferCell separation buffer
Overview
The EasySep™ Human Naïve CD4+ T Cell Isolation Kit II is designed to isolate naïve CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This kit replaces the EasySep™ Human Naïve CD4+ T Cell Enrichment Kit (Catalog #19155) and EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) for even faster cell isolations.
This kit replaces the EasySep™ Human Naïve CD4+ T Cell Enrichment Kit (Catalog #19155) and EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) for even faster cell isolations.
Advantages
• Fast, easy-to-use and column-free
• Up to 98% purity
• Untouched, viable cells
• Up to 98% purity
• Untouched, viable cells
Components
- EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Catalog #17555)
- EasySep™ Human Naïve CD4+ T Cell Isolation Cocktail II, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II (Catalog #17555RF)
- EasySep™ Human Naïve CD4+ T Cell Isolation Cocktail II, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
RoboSep™ Human Naïve CD4+ T Cell Isolation Kit II
|
Catalog #
17555RF |
Lot #
All |
Language
English |
Educational Materials (9)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Data and Publications
Data
Starting with fresh mononuclear cells, the naïve CD4+ T cell content (CD3+CD4+CD45RA+CD45RO-) of the isolated fraction is typically 96.6 ± 1.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 13.0% and 97.3%, respectively.
Publications (1)
Cell systems 2020 mar
Gut-Liver Physiomimetics Reveal Paradoxical Modulation of IBD-Related Inflammation by Short-Chain Fatty Acids.
Abstract
Abstract
Although the association between the microbiome and IBD and liver diseases is known, the cause and effect remain elusive. By connecting human microphysiological systems of the gut, liver, and circulating Treg and Th17 cells, we created a multi-organ model of ulcerative colitis (UC) ex vivo. The approach shows microbiome-derived short-chain fatty acids (SCFAs) to either improve or worsen UC severity, depending on the involvement of effector CD4 T cells. Using multiomics, we found SCFAs increased production of ketone bodies, glycolysis, and lipogenesis, while markedly reducing innate immune activation of the UC gut. However, during acute T cell-mediated inflammation, SCFAs exacerbated CD4+ T cell-effector function, partially through metabolic reprograming, leading to gut barrier disruption and hepatic injury. These paradoxical findings underscore the emerging utility of human physiomimetic technology in combination with systems immunology to study causality and the fundamental entanglement of immunity, metabolism, and tissue homeostasis.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
