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EasySep™ Human ILC2 Isolation Kit

Immunomagnetic positive selection kit using particle release technology

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human ILC2 Isolation Kit

Immunomagnetic positive selection kit using particle release technology

From: 1,268 USD
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Immunomagnetic positive selection kit using particle release technology
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Product Advantages


  • Easy-to-use and column-free

  • Up to 95% purity

  • Facilitates rapid isolation of ILC2s without flow sorting

  • No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

  • EasySep™ Human ILC2 Isolation Kit (Catalog #17782)
    • EasySep™ Human CRTH2-PE Positive Selection Cocktail, 0.1 mL
    • EasySep™ Release PE Positive Selection Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer, 1 mL
    • EasySep™ Human ILC2 Isolation Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
    • Anti-Human CD32 Blocker, 1 mL
 
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.

First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.

Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
 
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep™ Human ILC2 Isolation Profile

Starting with fresh leukapheresis samples, the ILC2 content (CD45+Lin- CRTH2+CD161+CD127+) of the isolated fraction typically ranges from 84 - 95%. In the above example, the purities of the start and final isolated fractions are 0.03% and 91% (gated on CD45), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
1000063935 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
1000063934 or lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
EasySep™ Human ILC2 Isolation Kit
Catalog #
17782
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Innate Lymphoid Cells
Wallchart
Innate Lymphoid Cells
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Easy Isolation of Particle-Free Human ILC2s from Peripheral Blood Mononuclear Cells
Scientific Poster
Easy Isolation of Particle-Free Human ILC2s from Peripheral Blood Mononuclear Cells

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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