EasySep™ Human ILC2 Isolation Kit
Immunomagnetic positive selection kit using particle release technology
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Required Products
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EasySep™ BufferCell separation buffer
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"The Big Easy" EasySep™ MagnetMagnet for column-free immunomagnetic separation
Overview
The EasySep™ Human ILC2 Isolation Kit is designed to isolate Group 2 Innate Lymphoid Cells (ILC2s) from leukapheresis products by immunomagnetic positive selection without the use of a flow sorter.
First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.
Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
First, CRTH2+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CRTH2+ cells, and unwanted non-ILC2s are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified ILC2s that are immediately ready for downstream applications.
Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages
• Easy-to-use and column-free
• Up to 95% purity
• Facilitates rapid isolation of ILC2s without flow sorting
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Up to 95% purity
• Facilitates rapid isolation of ILC2s without flow sorting
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
- EasySep™ Human ILC2 Isolation Kit (Catalog #17782)
- EasySep™ Human CRTH2-PE Positive Selection Cocktail, 0.1 mL
- EasySep™ Release PE Positive Selection Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™ 50201, 1 mL
- EasySep™ Release Buffer, 1 mL
- EasySep™ Human ILC2 Isolation Cocktail, 0.5 mL
- EasySep™ Dextran RapidSpheres™ 50103, 1 mL
- Anti-Human CD32 Blocker, 1 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet 1
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
1000063934 or lower |
Language
English |
|
Document Type
Product Information Sheet 2
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
1000063935 or higher |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 3
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 4
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 5
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 6
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 7
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 8
|
Product Name
EasySep™ Human ILC2 Isolation Kit
|
Catalog #
17782 |
Lot #
All |
Language
English |
Educational Materials (9)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Innate Lymphoid Cells
Wallchart
Human Immune Cytokines
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Scientific Poster
Easy Isolation of Particle-Free Human ILC2s from Peripheral Blood Mononuclear Cells
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
Innate Lymphoid Cell Research
Data and Publications
Data
Figure 1. Typical EasySep™ Human ILC2 Isolation Profile
Starting with fresh leukapheresis samples, the ILC2 content (CD45+Lin- CRTH2+CD161+CD127+) of the isolated fraction typically ranges from 84 - 95%. In the above example, the purities of the start and final isolated fractions are 0.03% and 91% (gated on CD45), respectively.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
