EasySep™ Human EpCAM Positive Selection Kit II
Immunomagnetic positive selection kit
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EasySep™ Human EpCAM Positive Selection Kit II -
EasySep™ Human EpCAM Positive Selection Kit II -
Label for EasySep™ Human EpCAM Positive Selection Kit II
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Required Products
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HBSS with 10 mM HEPES, Without Phenol Red
Hanks' Balanced Salt Solution (HBSS) modified with 10 mM HEPES, without phenol red -
Magnet for column-free immunomagnetic separation -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.
This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
• Up to 99% purity
• No columns required
Components:
- EasySep™ Human EpCAM Positive Selection Kit II (Catalog #17846)
- EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- RoboSep™ Human EpCAM Positive Selection Kit II with Filter Tips (Catalog #17846RF)
- EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Mammary Cells
Species:
Human
Sample Source:
Other; Primary
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Cancer Research; Epithelial Cell Biology
Related Products
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For culture of human mammospheres and tumorspheres -
For culture of human mammary epithelial cells -
For culture and evaluation of human mammary epithelial cells in CFU assays
Labeling Antibodies
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Anti-Human Keratin 18 Antibody, Clone DC-10
Mouse monoclonal IgG1 antibody against human keratin 18
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.