EasySep™ Human EpCAM Positive Selection Kit II

Immunomagnetic positive selection kit

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From: 673 USD

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Immunomagnetic positive selection kit
From: 673 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.

This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
Components:
  • EasySep™ Human EpCAM Positive Selection Kit II (Catalog #17846)
    • EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • RoboSep™ Human EpCAM Positive Selection Kit II with Filter Tips (Catalog #17846RF)
    • EasySep™ Human EpCAM Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Mammary Cells
Species:
Human
Sample Source:
Other; Primary
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Cancer Research; Epithelial Cell Biology

Scientific Resources

Product Documentation

Educational Materials

(5)
Brochure
EasySep™ Cell Separation Technology
Brochure
Mammary Epithelial Cells: Standardized Media and Reagents
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Scientific Poster
Column-Free Enrichment of EPCAM Positive and CD49F Positive Human Mammary Epithelial Progenitor Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Mammary Epithelial Cell Research
Tissue Dissociation and Isolation
Cell Culture and Assay
Characterization

Data and Publications

Data

Starting with peripheral blood mononuclear cells (PBMCs) seeded with MCF-7 cells (breast cancer cell line) at a starting frequency of 10.8 - 50.0%, the EpCAM+ cell content (epithelial cell+CD45-)of the isolated fraction is typically 96.2 ± 3% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.8% and 95.0%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.