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Due to pandemic-related plasticware shortages, you may see temporary changes to product packaging. Product quantity and quality are unaffected.

EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

From: 692 USD
Catalog #
17878_C
Immunomagnetic positive selection cell isolation kit

Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit (Catalog #17878)
    • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep™ Dextran RapidSpheres 50100, 2 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit with Filter Tips (Catalog #17878RF)
    • EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
    • EasySep™ Dextran RapidSpheres, 50100, 2 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.

This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
Monocytes
Species
Human
Sample Source
Buffy Coat
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology

Data Figures

Starting with buffy coat, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.9% and 98.4%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet - Buffy Coat
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Product Information Sheet - Buffy Coat
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit
Catalog #
17878RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (12)

HLA Antibodies for Purity Assessment
Brochure
HLA Antibodies for Purity Assessment
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subse...

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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