Overview
The EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.
This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
This product replaces the EasySep™ Human Buffy Coat CD14 Positive Selection Kit (Catalog #18088) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
• Up to 99% purity
• No columns required
Components:
- EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit (Catalog #17878)
- EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
- EasySep™ Dextran RapidSpheres 50100, 2 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ HLA Chimerism Buffy Coat CD14 Positive Selection Kit with Filter Tips (Catalog #17878RF)
- EasySep™ HLA Chimerism Buffy Coat CD14 Positive Selection Cocktail, 2 x 0.75 mL
- EasySep™ Dextran RapidSpheres, 50100, 2 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Monocytes
Species:
Human
Sample Source:
Buffy Coat
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; Immunology
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
17878
All
17878
All
17878RF
All
17878RF
All
17878RF
All
17878RF
All
17878RF
All
Educational Materials
(12)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data

Starting with buffy coat, the CD14+ cell content of the isolated fraction is typically 93.0 ± 6.4% (mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 8.9% and 98.4%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.