ClonaCell™ Media for Hybridoma and CHO Cell Line Development

Liquid and Semi-Solid Media for Efficient Cell Cloning and Generation

Develop CHO cell line, hybridomas, and other cell types using the ClonaCell™ portfolio of innovative cell line-specific media, designed to provide optimal culture conditions. Available in both methylcellulose-based (semi-solid) and liquid form, ClonaCell™-CHO and ClonaCell™-HY formulations help you achieve a high probability of monoclonality and clonal diversity in just one step.

View all ClonaCell™ media and supplements, and download our brochure to help you choose the right product for your needs.

Use ClonaCell™ to:

  • Save time and resources with a proven semi-solid cloning method that overcomes the challenges of traditional limiting dilution cloning
  • Nurture and save finicky hybridomas generated from mouse, rat, human, rabbit and hamster cells
  • Increase subcloning efficiency during CHO cell line development

Research-stage biotechnology companies and antibodies service companies use ClonaCell™-HY to complete antibody development projects faster and deliver the best antibodies for each application.

Dr. M. Javad Aman, President and CSO, Integrated BioTherapeutics, Inc.

Why Use ClonaCell™?

  • Save time with semi-solid cloning. Don’t waste resources on empty cloning wells.
  • Superior cloning efficiency and growth of CHO and other cell lines, hybridomas and myelomas.
  • Base methylcellulose medium is easily customized for your cell type. Custom formulations, packaging, and additional sizes are available upon request.
  • Rigorous performance testing, ensuring lot-to-lot reproducibility.
  • Industry-recognized manufacturing expertise and unparalleled technical support.

Try ClonaCell™ in your own lab.

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ClonaCell™ for Hybridoma Generation and Expansion

ClonaCell™-HY Hybridoma Kit

ClonaCell™-HY Hybridoma Kit

Recommended Applications:

All steps of mouse monoclonal antibody production: cell fusion, semi-solid cloning/selection and expansion of hybridomas

Advantages:

  • Combines the hybridoma selection and cloning into one step
  • Single-cell derived hybridomas form visible discrete colonies in semi-solid medium for easy picking and screening
  • Rare clones not lost through overgrowth

ClonaCell™FLEX

ClonaCell™FLEX

Size:

45 mL

Format:

Semi-solid

Formulation:

  • Serum-free
  • Chemically defined
  • Animal component-free
  • Protein-free
  • Methylcellulose
  • DHFR and GS compatible

Recommended Applications:

  • Creating customized semi-solid media for selection and cloning of suspension-adapted CHO cells and hybridomas
  • Converting a liquid medium into semi-solid medium for selection and cloning of a wide variety of cell types
  • Recloning of established, suspension-adapted CHO cells

Advantages:

  • Allows users to create a customized semi-solid medium optimized for a variety of cell types
  • Allows users to convert an existing liquid cloning method into a semi-solid cloning method

ClonaCell™-HY Liquid HAT Selection Medium

ClonaCell™-HY Liquid HAT Selection Medium

Size:

500 mL

Format:

Liquid

Formulation:

  • DMEM
  • Pre-selected serum
  • L-Glutamine
  • Gentamycin
  • Supplements
  • Hypoxanthine
  • Aminopterin
  • Thymidine
  • 2-Mercaptoethanol

Recommended Applications:

  • Selection of hybridomas following cell fusion
  • Cloning of individual hybridomas by limiting dilution

Advantages:

Simplifies hybridoma selection in liquid suspension cultures.

ClonaCell™-HY Medium D without HAT

ClonaCell™-HY Medium D without HAT

Size:

90 mL

Format:

Semi-solid

Formulation:

  • DMEM
  • Pre-selected serum
  • L-Glutamine
  • Gentamycin
  • Supplements
  • Hypoxanthine
  • Aminopterin
  • Thymidine
  • 2-Mercaptoethanol

Recommended Applications:

  • One-step selection (if appropriate selective agent is added) and semi-solid cloning of hybridomas
  • Used after cell fusion

Advantages:

  • Combines the hybridoma selection and cloning into one step
  • Single-cell derived hybridomas form visible discrete colonies in semi-solid medium for easy picking, screening, and expanding
  • Rare clones not lost through overgrowth

ClonaCell™-HY AOF Expansion Medium

ClonaCell™-HY AOF Expansion Medium

Size:

500 mL

Format:

Liquid

Formulation:

  • Hypoxanthine
  • Thymidine
  • Gentamicin
  • L-Glutamine
  • Recombinant proteins

Recommended Applications:

  • Growth and expansion of hybridomas (mouse, rat) and myelomas (mouse, rat, human)

Advantages:

  • Animal origin-free
  • Reduces performance variability in the medium
  • Simplifies downstream clone screening and antibody purification (no serum-derived IgG)
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ClonaCell™-HY Hybridoma Kit Media

ClonaCell™-HY Medium A

ClonaCell™-HY Medium A

Size:

500 mL

Format:

Liquid

Ingredients:

  • DMEM
  • Pre-selected serum
  • L-Glutamine
  • Gentamycin
  • Supplements
  • 2-Mercaptoethanol

Application:

Supports the growth of myeloma cells prior to fusion

ClonaCell™-HY Medium B

ClonaCell™-HY Medium B

Size:

500 mL

Format:

Liquid

Ingredients:

  • DMEM
  • L-Glutamine
  • Gentamycin
  • 2-Mercaptoethanol

Application:

Used during the fusion process

ClonaCell™-HY Medium C

ClonaCell™-HY Medium C

Size:

100 mL

Format:

Liquid

Ingredients:

  • DMEM
  • Pre-selected serum
  • L-Glutamine
  • Gentamycin
  • Supplements
  • 2-Mercaptoethanol

Application:

Promotes hybridoma viability after fusion prior to HAT selection

ClonaCell™-HY Medium D

ClonaCell™-HY Medium D

Size:

90 mL

Format:

Semi-solid

Ingredients:

  • DMEM
  • Pre-selected serum
  • L-Glutamine
  • Gentamycin
  • Supplements
  • Hypoxanthine
  • Aminopterin
  • Thymidine
  • 2-Mercaptoethanol

Application:

Used for selection and cloning of hybridomas in one step

ClonaCell™-HY Medium E

ClonaCell™-HY Medium E

Size:

500 mL

Format:

Liquid

Ingredients:

  • DMEM
  • Pre-selected serum
  • L-Glutamine
  • Gentamycin
  • Supplements
  • Hypoxanthine
  • Thymidine
  • 2-Mercaptoethanol

Application:

Supports hybridoma growth and expansion after HAT selection
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ClonaCell™ for CHO Cell Line Development

ClonaCell™-CHO CD Medium

Size:

90 mL

Format:

Semi-solid

Formulation:

  • Serum-free
  • Chemically defined
  • Animal component-free
  • Protein-free
  • DHFR and GS compatible

Ingredients:

  • Methylcellulose
  • Kolliphor P188 / Pluronic F68

Recommended Applications:

  • Selection and cloning of suspension-adapted CHO cells
  • Recloning of established, suspension-adapted CHO cells

Advantages:

Enables single-cell cloning in a chemically defined, animal component-free, protein-free medium

ClonaCell™ FLEX

ClonaCell™ FLEX

Size:

45 mL

Format:

Semi-solid

Formulation:

  • Serum-free
  • Chemically defined
  • Animal component-free
  • Protein-free
  • DHFR and GS compatible

Ingredients:

Methylcellulose

Recommended Applications:

  • Creating customized semi-solid media for selection and cloning of suspension-adapted CHO cells and hybridomas
  • Converting a liquid medium into semi-solid medium for selection and cloning of a wide variety of cell types
  • Recloning of established, suspension-adapted CHO cells

Advantages:

  • Allows users to create a customized semi-solid medium optimized for a variety of cell types
  • Allows users to convert an existing liquid cloning method into a semi-solid cloning method

ClonaCell™-CHO CD Liquid

ClonaCell™-CHO CD Liquid

Size:

500 mL

Format:

Liquid

Formulation:

  • Serum-free
  • Chemically defined
  • Animal component-free
  • Protein-free
  • DHFR and GS compatible

Ingredients:

Kolliphor P188 / Pluronic F68

Recommended Applications:

  • Culture of suspension-adapted CHO cells
  • Adaptation of CHO cells for selection and cloning
  • Expanding CHO cells following semi-solid cloning
  • Limiting dilution cloning of CHO cells when used with ClonaCell™-CHO ACF Supplement
  • Cryopreservation of CHO cells when combined with DMSO

Advantages:

  • Supports expansion of CHO cells in a chemically defined, animal component-free, protein-free medium
  • Compatible with all other ClonaCell™ products

ClonaCell™-CHO ACF Medium

Size:

90 mL

Format:

Semi-solid

Formulation:

  • Serum-free
  • Animal component-free
  • DHFR and GS compatible

Ingredients:

  • Methylcellulose
  • Supplements
  • Kolliphor P188 / Pluronic F68

Recommended Applications:

  • Selection and cloning of transfected suspension-adapted CHO cells where serum-free or animal component-free media is required
  • Recloning of established, suspension-adapted CHO cells

Advantages:

  • Contains only recombinant proteins and synthetic components
  • Greater single cell cloning efficiency of CHO cells compared with chemically defined, protein-free media (particularly at low plating densities)

ClonaCell™-CHO ACF Supplement

ClonaCell™-CHO ACF Supplement

Size:

2.5 mL

Format:

Liquid

Formulation:

  • Serum-free
  • Animal component-free
  • DHFR and GS compatible

Ingredients:

Supplements

Recommended Applications:

  • Supplementing chemically defined, protein-free media or semi-solid media to promote growth of CHO cells during single-cell cloning and expansion
  • Supporting CHO cell expansion

Advantages:

  • Contains only recombinant proteins and chemically defined components at 40X
  • Does not contain serum or hydrolysates

ClonaCell™-TCS Medium

ClonaCell™-TCS Medium

Size:

80 mL

Format:

Semi-solid

Formulation:

Serum-containing

Ingredients:

  • Methylcellulose
  • 2-Mercaptoethanol
  • L-Glutamine

Recommended Applications:

  • Selection, cloning, and recloning of suspension-adapted or adherent CHO cells and other cell lines
  • Applications where high cloning efficiency and robust colony formation is important

Advantages:

Supports high cloning efficiency and robust colony formation for a variety of cell lines such as: CHO-K1, CHO-S, HEK-293, B16F-10, BaF/3, BHK-1, FD-5, Jurkat Daudi, Molt-4, UT-7
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Scientific Resources

Key Applications

Cell Line Development:

Ling SSM et al. (2015) Instrumental Role of Helicobacter Pylori γ-Glutamyl Transpeptidase in VacA-Dependent Vacuolation in Gastric Epithelial Cells. PLoS One 10(6): e0131460.
Rodríguez L et al. (2015) Generation of Monoclonal Antibodies Specific of The Postfusion Conformation of The Pneumovirinae Fusion (F) Protein. J Virol Methods.
Young J et al. (2015) A Novel Immunoassay to Measure Total Serum Lymphotoxin-α Levels in The Presence of An Anti-LTα Therapeutic Antibody. J Immunol Methods Epub ahead.
Chronopoulou E et al. (2014) Hybridoma Technology for The Generation of Rodent mAbs via Classical Fusion. In: Ossipow V, N Fischer (Eds.) Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology. 2nd Ed. New York: Springer Science+Business Media.: pp47–70.
Date Y et al. (2014) Label-Free Impedimetric Immunoassay for Trace Levels of Polychlorinated Biphenyls in Insulating Oil. Anal Chem 86(6): 2989–2996.
García-Barreno B et al. (2014) Characterization of An Enhanced Antigenic Change in The Pandemic 2009 H1N1 Influenza Virus Haemagglutinin. J Gen Virol 95(PART 5): 1033–1042.
Tan GS et al. (2014) Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets The Fusion Domain of Group 2 Influenza A Virus Hemagglutinin. J Virol 88(23): 13580–13592.
Kelly-Cirino CD & Mantis NJ. (2009) Neutralizing Monoclonal Antibodies Directed Against Defined Linear Epitopes on Domain 4 of Anthrax Protective Antigen. Infect Immun 77(11): 4859–4867.
Weidanz J a et al. (2006) Levels of Specific Peptide-HLA Class I Complex Predicts Tumor Cell Susceptibility to CTL Killing. J Immunol 177(8): 5088–5097.
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