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ClonaCell™ FLEX

Semi-solid methylcellulose-based medium for selecting and cloning cells

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ClonaCell™ FLEX

Semi-solid methylcellulose-based medium for selection and cloning of cells

45 mL
Catalog #03818
246 USD

Overview

ClonaCell™ FLEX is a base methylcellulose medium that requires the addition of a 2X concentrate of the user’s own liquid medium to create a custom semi-solid cloning medium. This allows the user to adapt an existing liquid cloning method to a customized semi-solid cloning method. ClonaCell™ FLEX is recommended for selection and cloning of suspension-adapted mammalian cells, including CHO cells and hybridomas. The medium is chemically defined, animal component-free, and protein-free. It does not contain L-glutamine, selection agents, or phenol red. ClonaCell™ FLEX is compatible with a wide range of selection systems, including dihydrofolate reductase (DHFR), glutamine synthetase (GS), and hypoxanthine, aminopterin, thymidine (HAT).

Benefits of semi-solid cloning:
• Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening.
• Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning.
• Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion.
Advantages:
• Allows the user to convert an existing liquid cloning method into a semi-solid cloning method using a 2X concentrate of their liquid medium of choice
• Flexibility to create a custom semi-solid medium optimized for cloning a wide variety of mammalian or non-mammalian cell types
Contains:
• Methylcellulose
• Water
Subtype:
Semi-Solid Media; Specialized Media
Cell Type:
CHO Cells; HEK-293; Hybridomas; Other
Species:
Mouse; Other
Application:
Cell Culture; Semi-Solid Cloning
Brand:
ClonaCell
Area of Interest:
Antibody Development; Cell Line Development; Drug Discovery and Toxicity Testing; Hybridoma Generation
Formulation:
Animal Component-Free; Serum-Free; Chemically Defined; Protein-Free

Scientific Resources

Product Documentation

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Educational Materials

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Frequently Asked Questions

Is the serum in ClonaCell™-TCS medium heat inactivated?

Yes, all serum used in ClonaCell™ is heat inactivated.

Is there any IgG in ClonaCell™ TCS?

While we don't add IgG to the ClonaCell™ media, we do add serum, which contains an undefined amount of IgG. We selectively use serum lots with low IgG levels in the production of ClonaCell™ media, however, levels vary from lot to lot. IgG levels in a specific lot of ClonaCell™ TCS medium are available in the lot-specific Certificate of Analysis.

Can ClonaCell™-TCS be used with any cell line?

A list of recommended cell lines can be found in the manual. Other cell lines may be compatible with ClonaCell™-TCS. It will be necessary, however, to determine the plating cell density and growth efficiency of the desired cells in ClonaCell™-TCS.
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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.