ClonaCell™-HY Medium E
Hybridoma growth medium with hypoxanthine and thymidine (serum-containing)
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Overview
• Serum
• Hypoxanthine and thymidine (HT)
• Gentamicin
• 2-Mercaptoethanol
• Phenol red
• L-Glutamine and other supplements
• Other ingredients
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (9)
Frequently Asked Questions
Why is there HT (hypoxanthine, thymidine) in Medium E?
Is the serum in ClonaCell™-HY media heat inactivated?
Is there any IgG in clonacell™-HY media?
Are there antibiotics in ClonaCell™-HY media?
What is the optimal number of colonies per plate?
Why do I have to put my fused cells into liquid medium overnight? Why can't I just plate directly into Medium D?
What myeloma and mouse strains should I use?
Can I grow human/rat/T cell hybridomas in ClonaCell™-HY?
There are very few colonies growing in my Medium D. Why?
Why does the ClonaCell™-HY manual suggest two different methods for fusion (A or B)? Can one expect better results with one method over the other?
Why does the ClonaCell™-HY manual suggest two different methods for fusion (A or B)? Can one expect better results with one method over the other?
Once I pick the colonies and grow the cells in plates, will the residual methylcellulose interfere with characterization? For example, will I have problems doing an ELISA?
Is the serum in ClonaCell™-TCS medium heat inactivated?
Is there any IgG in ClonaCell™ TCS?
Can ClonaCell™-TCS be used with any cell line?
Why do I get more cells when I select my fusion in liquid medium rather than in methylcellulose-based semi-solid medium?
How do I thaw ClonaCell™ methylcellulose-based semi-solid medium?
How do I measure and dispense methylcellulose semi-solid medium?
My ClonaCell™ methylcellulose semi-solid medium appears runny. Why does this happen?
What is the optimal number of colonies per plate?
There are still bubbles in the media after I plate my cells. Do I need to disrupt the bubbles?
Do I ever need to re-clone cultures grown with ClonaCell™ semi-solid medium?
Once I pick the colonies and grow the cells in plates, will the residual methylcellulose interfere with characterization? For example, will I have problems doing an ELISA?
How important is the incubator humidity when culturing in methylcellulose-based medium?
Do I have to use 100 mm petri dishes or can I use other cultureware?
Publications (2)
Abstract
Abstract
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.