Benefits of semi-solid cloning:
- During semi-solid cloning, individual cells are suspended in viscous medium, which physically separates them and allows them to grow into discrete colonies that can be easily isolated.
- With semi-solid cloning, monoclonal cell lines can be isolated in less time and using fewer resources than with limiting dilution cloning.
- It is easy to isolate rare, high-producing clones, compared with selection in bulk liquid medium. This is because diverse clones with a wide range of growth rates and productivities form discrete colonies.